硬质干酪对维甲酸诱导大鼠骨质疏松防护效果的研究

STUDY ON THE PROTECTIVE EFFECTS OF CHEESE ON OSTEOPOROTIC RATS INDUCED BY RETINOIC ACID

目的观察硬质干酪对大鼠骨质疏松的防护作用。方法 SD大鼠48只,随机分为正常对照组、模型组、干酪高、中、低剂量组和葡萄糖酸钙对照组,每组各8只。除正常组外,其余各组用维甲酸按80mg/(kg.d)连续灌胃建立骨质疏松模型,并灌服相应的受试物和药物,正常组和模型组灌胃给予同等体积蒸馏水。实验结束后处死大鼠,观察骨密度、血清生化、骨矿含量和骨形态计量学的变化。结果模型组左侧股骨骨密度、骨矿含量、骨小梁面积百分比和平均骨小梁宽度均明显减少。血清钙、磷、碱性磷酸酶活性、抗酒石酸酸性磷酸酶含量和破骨细胞均明显升高,提示骨质疏松模型复制成功。干酪高剂量组左侧股骨密度、骨矿含量、抗酒石酸酸性磷酸酶含量与正常对照组、葡萄糖酸钙组比较无明显差异。结论通过补充硬质干酪增加钙的摄入,可提高大鼠的骨密度、骨钙、磷含量,对骨质疏松有一定的预防保护作用。

Objective To study the protective effect of cheese on osteoporotic rats induced by retinoic acid.Method Forty-eight SD rats were randomly divided into normal group,model group,high,middle and low dose cheese group,gluconate calcium group.All rats except the nomal group were treated with oral retinoic acid 80mg/（kg？d）.The normal group and model group were given the same dose of distilled water.At the end of experiment,the rats were killed to measure the concentrations of serum calcium,phosphorus,alkaline phosphatase（ALP）,tartrate resistant acid phosphatase（TRACP）,bone mineral density（BMD） and bone mineral content（BMC） and the morphological changes of femurs.Results As compared with the nomal group,BMD,BMC,trabecular area percent（Tb.Ar%） and mean trabecular plate thickness（MTPT） of the left femur in the model group were obviously decreasd,while serum levels of calcium,ALP and TRACP were significantly increased,suggesting osteoporosis.BMD,BMC,Tb.AR%,MTPT and TRACP did not differ significantly between high dose cheese group and the normal group.Conclusion The dietary hard cheese supplement can increase the BMD and BMC of the rats,suggesting the protective effect on osteoporosis.