双歧杆菌在不同材料上形成生物膜的研究

Study on Biofilm Formation of Bifidobacterium Sp. on Different Materials

目的:评价双歧杆菌生物膜生成能力。方法:采用结晶紫染色和刚果红染色等方法对几种不同双歧杆菌形成生物膜的能力进行测定和鉴定。结果:采用不同途径分离的9株双歧杆菌分别在亲水性材料和疏水性材料上展现不同的生物膜形成能力。所有菌株在聚丙烯材料上均表现出弱的生物膜形成能力,而在聚苯乙烯和硼硅酸盐材料表面均表现出不同程度的生物膜形成能力。利用盖玻片对生物膜的显微观察表明生物膜含有多糖成分。细胞表面疏水性研究表明双歧杆菌中生物膜的形成与细胞表面疏水性未见相关性。结论:双歧杆菌具有一定的生物膜生成能力。

Objective：Biofilm formation ability of bifidobacterium was evaluated.Methods：Biofilm formation ability of several different Bifidobacteriums was determined and identified by crystal violet and Congo red staining methods.Results：The 9 isolates exhibited different capacities of biofilm formation on hydrophilic and hydrophobic material surfaces.All isolates formed weak biofilms in polypropylene （PP） tubes.While strong,moderate or weak biofilms were detected in borosilicate（BS） tubes or polystyrene （PS） microplates.The microscopic analysis of biofilms formed on glass coverslips revealed that the biofilm contains polysaccharides exopolysaccharides.Poor correlation was observed between cell surface hydrophobicity and biofilm formation.Conclusion：Bifidobacterium sp.have biofilm formation ability to a certain degree.