多烯磷脂酰胆碱对β-淀粉样蛋白(Aβ1-40)致阿尔茨海默病模型大鼠的治疗作用

Polyene Phosphatidyl Choline for Alzheimer's Disease Model Rats Induced by β-Amyloid Protein(1-40)

目的:研究多烯磷脂酰胆碱(Polyene Phosphatidyl choline,PPC)对β-淀粉样蛋白(Aβ1-40)致阿尔茨海默病(Alzheimer's Dis-ease,AD)模型大鼠的治疗作用。方法:32只SD大鼠随机分为正常组、假手术组、模型组和处理组,海马内注射淀粉样蛋白(Aβ1-40),制作大鼠阿尔茨海默病模型,处理组给予多烯磷脂酰胆碱。通过Morris水迷宫实验验检测各组大鼠认知行为学改变,海马组织学及免疫组化染色,观察多烯磷脂酰胆碱对阿尔茨海默病模型大鼠的影响,并进行统计学分析。结果:①Morris水迷宫实验,模型组与正常组、假手术组比较,学习和记忆潜伏期显著增加;处理组与模型组比较,学习和记忆潜伏期显著减少;组间比较差异有统计学意义(P<0.05),提示多烯磷脂酰胆碱使AD模型大鼠的认知行为学功能改善。②Nissl染色,模型组与正常对照组、假手术组组织学染色结果有显著性差异(P<0.05),PPC处理组与模型组比较有显著性差异(P<0.05),PPC处理组与正常组及假手术组比较也呈现出显著性差异(P<0.05);提示多烯磷脂酰胆碱可以减少神经元的凋亡。③β-淀粉样蛋白免疫组化染色,模型组与正常对照组、假手术组比较,Aβ沉积明显增加;PPC处理组与模型组比较,Aβ沉积范围明显缩小,PPC处理组与正常对照组、假手术组也呈现出显著性差。结论:多烯磷脂酰胆碱对淀粉样蛋白(Aβ1-40)致阿尔茨海默病模型大鼠有治疗作用。

Objective： To investigate the therapeutical effects of Polyene Phosphatidyl Choline（PPC） on β-amyloid（1-40） induced Alzheimer＇s Disease model Rat. Methods： Thirty-two Sprague-Dawley rats were divided into four groups： normal control group, sham control group, model group and PPC treatment group, β-Amyloid protein was infused into the hippocampal fissure of rats to produce Alzheimer＇s Disease （AD） model and the treatment group rats were given PPC. Morris water maze was detected to evaluate the cognition behavior of learning and memory. The histological and immunohistochemistry staining were used to observe the hippocampus changes caused by PPC treatment in the AD model rats. Results： （1）Compared that in the normal control group and sham control group, learning and memory latency of the model group was prolonged significantly （P 〈0.05）; As it to PPC treatment group, compared with Model group, learning and memory latency was significantly shortened （P〈0.05）; The differences between groups were statistically significant （P〈0. 05）. （2） Nissl staining： compared the model group with the normal control group, sham control group, the results were significantly differences （P 〈0.05）; compared the PPC treatment group with the model group , the result was significantly different （P 〈0.05）; comparing the treatment group with the normal control group and the sham control group, the results also show significant difference （P〈 0.05）. （3）Aβimmunohistochemical staining： Compared with normal control group and sham control group, the model group show obvious increasing amount ofβ-amyloid deposits; Compared with model group, the PPC treatment group indicate significant decreasing of the number ofβ-amyloid deposits. Conclusion： Polyene phosphatidyl Choline had therapeutical effects on Alzheimer＇s Disease model rats induced by β-amyloid protein （1-40）.