HSP70和P53蛋白在甲状腺乳头状癌中的表达及其与临床病理特征的关系

Expression of HSP70 and p53 and Their Relationship with Pathological Characteristics in Papillary Thyroid Carcinoma

目的:探讨热休克蛋白70(HSP70)和P53蛋白在甲状腺乳头状癌组织中的表达及其与临床病理特征的关系。方法:应用免疫组织化学Envision法检测35例甲状腺乳头状癌组织、15例甲状腺良性病变组织及15例正常甲状腺组织中HSP70和P53蛋白的表达,分析HSP70和P53蛋白的表达与甲状腺乳头状癌临床病理学特征的关系。结果:甲状腺乳头状癌组织中HSP7O和P53阳性表达率明显高于甲状腺良性病变组织及正常甲状腺组织(P<0.01)。HSP7O在甲状腺乳头状癌组织中的表达与是否有颈部淋巴结转移、浸润深度以及AJCC分期密切相关(P<0.01),而与年龄、性别、肿瘤的大小以及分化程度无关(P>0.05)。P53在PTC组织中的表达与组织分化程度、是否有淋巴结转移、浸润深度以及AJCC分期密切相关(P<0.01),而与年龄、性别、肿瘤的大小无关(P>0.05)。HSP70和P53蛋白在PTC组织中的表达呈正相关性(r=0.679,P<0.01)。结论:HSP70和p53蛋白在PTC中均呈高表达,并有协同作用,两者可作为预测PTC的生物学行为和预后的参考指标。

Objective： To investigate the relationship of the expression of HSP70 and p53 with the pathological characteristic in papillary thyroid carcinoma. Methods： The expression of HSP70 and p53 protein were detected with immunohistochemical EnVision plus non-hiotin technique in 35 cases of papillary thyroid carcinoma,15 cases of benign thyroid diseases and 15 eases of normal tissues of thyroid. Results： The positive expression rates of HSP70 and p53 protein in papillary thyroid carcinoma were significantly higher than that in benign thyroid diseases and normal tissues of thyroid （P〈0.01）. The expression of HSP70 was positively related to lymph node metascasis, AJCC clinical stage and the degree of infiltration （P〈0.01）. The expression of p53 was positively related to AJCC clinical stage, differentiation degree, lymph node metascasis and the degree of infiltration （P〈0.01）. The expression of HSP70 was positiyely correlated with p53 （r=0.679,P〈0.01）. Conclusion： The expression of liSP70 and p53 protein in papillary thyroid carcinoma were high. There was the synergistic effect between HSP70 and p53. HSP70 and p53 protein may act as markers predicting biological behavior and prognosis of papillary thyroid carcinoma.