摘要
目的:建立测定酸枣仁汤中甘草苷含量的反相高效液相色谱分析方法。方法:色谱柱:WatersC18(250nm×4.6mm,5μm);流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;流速:1.0ml/min,检测波长:276nm。结果:芒果苷在0.093μg-0.744μg范围内与峰面积线性关系良好(r=0.9999),平均加样回收率为98.04%,RSD为0.41%。结论:本方法简便、准确、可靠、重复性好,可作为酸枣仁汤中甘草苷的含量测定方法。
Objective： To establish a HPLC method for the determination of Liquiritin in Suanzaoren Decoction. Methods：The determination was performed on WatersC 18 column （250nm ×4.6mm,5μm）with a grient mobile phase composed of acetionitrile and 0.1% phosphoric acid. The detection wavelength was 276nm,and the flow rate was lml/min.Results：The Mangiferin showed good linearity （r=0.9999）in the range of 0.093μg-0.744μg, the average recovery was 98.04% with RSD 0.41%. Conclusions：The method is simple, accurate, reliable and is suitable for Determination of Liquiritin in Suanzaoren Decoction.
出处
《现代生物医学进展》
CAS
2011年第13期2543-2545,共3页
Progress in Modern Biomedicine
基金
2008年教育部博士点基金(200802280001)
