PCV-2感染对PK-15细胞抗病毒相关因子转录时相的影响

Transcriptional profiles of inflammatory related cytokines in PK-15 cell cultures following infection with Porcine circovirus type 2

【目的】了解猪圆环病毒2型(PCV-2)感染对PK-15细胞抗病毒相关因子转录时相的影响,探讨宿主-病毒之间的作用关系。【方法】运用荧光定量PCR技术,测定和分析PCV-2感染PK-15细胞后0(未接种病毒),1,6,12,24,48,72 h时病毒DNA含量及细胞因子IFN-βI、FN-γI、FNAR-1I、FNAR-2、MHC-Ⅰ、MHC-Ⅱ、MX1、NOS、RNaseL和IRF-3 mRNA转录水平的变化。【结果】PCV-2感染PK-15后1 h就可以检测到病毒DNA,在感染后24 h病毒开始大量迅速增殖,于感染72 h时达到最高峰。与0 h相比,PCV-2感染后IFN-βI、RF-3的表达量在感染12 h时显著增加,其他时间表达量下降;IFN-γ、MHC-Ⅱ的表达量在感染12 h时增加不明显,其他时间表达量下降;感染PCV-2后IFNAR-2、MHC-Ⅰ、MX1的表达量下降;RNaseL的表达量在感染12 h时显著减少,其他时间表达量增加,48 h时表达量增加显著;IFNAR-1的表达量在感染72 h时显著增加,其他时间表达量下降;未检测出NOS的表达。【结论】随着PCV-2病毒含量的增加,以上几种主要抗病毒因子的表达量不明显或有所下降,表明PCV-2感染PK-15细胞后可逃避机体的抗病毒作用机制。

【Objective】 The study was to investigate the host inflammatory responses and the host-PCV-2 interaction in particular antiviral cytokines responses when the Porcine Kidney-15 cells（PK-15） were infected with Porcine Parvovirus（PCV-2）.【Method】 After the Porcine Kidney-15 cells（PK-15） were infected with PCV-2,the viral DNA was measured and analyzed by real-time PCR,so were the transcript level of cytokines（IFN-β,IFN-γ,IFNAR-1,IFNAR-2,MHC-Ⅰ,MHC-Ⅱ,MX1,NOS,RNaseL and IRF-3）.【Result】 We found that the transcript levels of IFN-β,IRF-3 were significantly increased at 12 h postinoculation（p.i.）,but decreased at other time.The transcript levels of IFN-γ,MHC-Ⅱ were slightly increased at 12 h p.i.and decreased at other time.The transcript levels of IFNAR-2,MHC-Ⅰ,MX1 were decreased.The transcript levels of RNaseL were significantly decreased at 12 h p.i.but increased at other time.The transcript levels of IFNAR-1 were significantly increased at 72 h p.i.but decreased at other time.【Conclusion】 With the increase of the content of the PCV-2,the expression quantities of the above several main antiviral factors were not obvious or declined which showed PCV-2 infection can evade the antiviral mechanism of PK-15 cells.