620nm低能量红光对骨髓间充质干细胞成骨分化的影响

620 nm Red Light Enhances Osteogenic Differentiation in Mesenchymal Stem Cells

为研究发光二极管(LED)发射的红光对大鼠骨髓间充质干细胞的成骨分化的影响,体外培养SD大鼠骨髓间充质干细胞,620nm波长的LED置于细胞上方2cm处,照射剂量分别为0,1,2和4J/cm^2。采用CCK-8法检测照射后第2、4天的增殖活性,碱性磷酸酶(ALP)活性试剂盒与von kossa矿化结节染色法检测骨髓间充值干细胞的成骨分化情况。结果表明在第4天,4J/cm^2的照射剂量致细胞的活性明显降低(P<0.05)并促进ALP活性明显增加(P<0.05),von kossa染色显示红光照射组的钙盐沉积较多。综上所述,620 nm LED非相干红光可有效促进骨髓间充质干细胞的成骨分化。

To investigate the effect of red light emitted from light-emitting diode （LED） on hone marrow Mesenchymal stem cells （MSCs） cultured with osteogenic supplements, MSCs were divided into four groups and each group was irradiated at dose of 0, 1, 2, and 4 J/cm^2. Cellular proliferation was evaluated by using CCK-8. The alkaline phosphatase （ALP） activity and mineralization formation were monitored as indicators of MSCs＇ differentiation toward osteoblasts. As a result, the cellular proliferation was decelerated in 4 J/cm^2 group, wherears red light increased ALP activity and mineralized nodule formation. In conclusion, noncoherent red light from LED can enhance osteogenic differentiation and decelerates proliferation of MSCs in media with osteogenic supplements.