地衣芽孢杆菌D-1产碱性蛋白酶培养条件的优化

Optimization of Culture Conditions of Alkaline Protease Production by Bacillus licheniformis D-1

为了对产碱性蛋白酶的地衣芽孢杆菌D．1的培养条件进行优化，利用10L发酵罐，采用正交设计L9（3^4）试验，对培养温度、pH值、搅拌转速、通气量4条件进行优化，得到地衣芽孢杆菌D-1发酵产碱性蛋白酶的最优培养条件为：培养温度37．0℃，pH值7．5，通气量4L/min，搅拌转速300r／min。利用最优条件组合进行验证实验表明，该培养条件下地衣芽孢杆菌D-1碱性蛋白酶的酶活为91．26U／mL。

In order to optimize culture condition for alkaline protease production by Bacillus licheniformis D-1, the culture temperature, pH, stirring speed and air flow rate during fermentation were optimized by the L9 （34） orthogonal test in 10 L bioreactor. The optimized culture conditions were determined as a culture temperature of 37 ℃ , a pH of 7, 5, an air flow rate 4 L/min, and a stirring speed of 300 r/min. Under these optimization conditions, the confirmative experiment shows that the activity of alkaline protease produced by Bacillus licheniformis D-1 reached 91.26 U/mL.