黑曲霉对黄曲霉生长、产毒及黄曲霉毒素B_1的影响

Influence of Aspergillus niger on growth of Aspergillus flavus and aflatoxin B_1

目的研究黑曲霉对黄曲霉生长、产毒的抑制作用及对AFB1的降解作用。方法将黑曲霉分别与黄曲霉、AFB1共同培养,定期测定培养液pH、菌丝体干重、黄曲霉孢子数、AFB1含量。结果黑曲霉与黄曲霉混合培养时,黄曲霉孢子数、AFB1含量均比单独培养的低,2组之间差异有统计学意义（P〈0.05）,抑制率达到68.06%-91.52%;加入黑曲霉后,AFB1含量降低,实验组与对照组之间差异有统计学意义（P〈0.05）,降解率为46.19%。结论黑曲霉既能抑制黄曲霉生长、产毒,又能降解AFB1。

Objective To study the influence of Aspergillus niger on the growth of Aspergillus flavus and degradation of AFB1.Method Aspergillus niger was co-cultured with Aspergillus flavus and AFB1,respectively;pH,dry weight of mycelium,spores of Aspergillus flavus and content of AFB1 were determined every 3 days.Result Compared with the control group,the number of Aspergillus flavus spores and content of AFB1 were much less in competitive situation.There was a significant difference between the two groups（P0.05） and the rate of inhibition was 68.06% to 91.52%;more-over,AFB1 was reduced when Aspergillus niger was inoculated,with is a significant difference between the two groups（P0.05）,and the rate of degradation was 46.19%.Conclusion Aspergillus niger not only can inhibit the growth of Aspergillus flavus and production of AFB1,but also degrade AFB1.