bcl-2、bax在砷中毒大鼠肾近端小管表达

Expression of bcl-2,bax in renal proximal tubular epithelial cells of rats with arsenic poisoning

目的探讨砷中毒对大鼠肾近端小管上皮细胞凋亡调控基因bc l-2、bax影响。方法清洁级SD大鼠40只,体重为120～150g,高、低剂量染砷组各15只,对照组10只。高、低剂量染砷组分别给予三氧化二砷(AS2O3)10、0.4 mg/kg水溶液自由饮用,对照组饮用蒸馏水。分笼喂养4个月,取肾脏标本,采用免疫组织化学二步法、细胞计数和图像分析方法测定bc l-2、bax表达。结果高、低剂量染砷组肾近端小管上皮bc l-2阳性细胞计数分别为(1.85±1.22)与(5.47±1.62)个,明显低于对照组(8.03±2.42)个,平均灰度值逐渐增高,差异具有统计学意义(P<0.05);高、低剂量染砷组肾近端小管上皮bax阳性细胞数分别为(14.88±3.02)与(6.89±1.86)个,明显高于对照组(2.18±1.52)个,平均灰度值随着染砷剂量的增加而下降,差异具有统计学意义(P<0.05)。结论 bc l-2、bax参与了砷中毒大鼠近端小管上皮细胞凋亡过程。

Objective To investigate the influence of arsenic poisoning on the expressions of bcl-2,bax apoptosis control gene in renal proximal tubular epithelial cells in rtas.Methods Forty normal SD rats were divided into high and low dose of arsenic poisoning group and control group.The body weights of the rats were 120-150g.There were 15 rats in high and low dose exposure groups,and 10 rats in the control group.The rats in high and low groups were treated with As2O3 through drinking water at the doses of 10 and 0.4 mg/kg·d.The control group was given distilled water.Four months after the treatment,the kidney tissue of the rats was collected.Two step immunohistochemistry method,cell number count,and image analyses were used in the study.Results The bcl-2 immunoractive cells decreased and the average gray value gradually increased in arsenic poisoning groups（P0.05）.The bax immunoractive cells of renal proximal tubular epithelial were increased and the average gray value decreased（P0.05） in arsenic poisoning groups compared to those of the control group.Conclusion The expression of bcl-2,bax apoptosis control gene are involved in the process of apoptosis of renal proximal tubular epithelial cells in arsenic poisoning rats.