3种硬组织替代材料骨细胞相容性研究

Cytocompatibility of Three Substituted Materials of Human Beings' Hard Tissue

目的 :研究羟基磷灰石 (HA)、生物玻璃陶瓷 (BGC)和钛合金 (Ti- 6Al- 4 V) 3种骨替代材料的细胞相容性 ,探讨体外评价骨替代材料细胞相容性的简捷快速的实验方法。方法 :选用 SD乳鼠颅顶骨成骨细胞 ,采用体外细胞培养方法 ,将细胞接种于 HA、BGC和 Ti- 6Al- 4 V 3种材料表面后 ,分别观察 1、3、5、7d时细胞形态及生长增殖率 ,评价 3种材料的骨细胞相容性。结果 :在 3种材料的表面 ,成骨细胞以锚状结构牢固地粘附 ,并具有良好的细胞形态和增殖率。其中以 HA和 BGC的骨细胞相容性较好 ,Ti- 6Al- 4 V相对较差。结论 :体外成骨细胞培养法从细胞形态及生长增殖率方面能客观反映 3种硬组织替代材料和细胞之间的相互作用 ,可作为体外研究骨替代材料细胞相容性的简捷快速的实验方法。

Objective: To evaluate the osteocompatibility of three substituted materials of human beings' hard tissue (hydroxyapatite, bioglass ceramic and Ti 6Al 4V) and a new experimental method for evaluating the osteocompatibility of replaced materials of hard tissues in vitro. Methods: The isolated osteoblasts from parietal and frontal bones of Sparague Dawley rats from 1 day to 3 days were used to estimate osteocompatibility of three substituted materials in vitro. The cells were inoculated onto specimens of HA, BGC and Ti 6Al 4V for 1 day, 3, 5 and 7 days, then the morphology and proliferating rates of cells on different materials were tested. Results: Rat osteoblasts could attach, spread and proliferate on three materials as normal cells, and proliferating rates of cells on HA and BGC surface were as the same as normal, but that on Ti 6Al 4V was lower than the normal cells. Conclusion: The osteocompatibility of HA and BGC are better than that of Ti 6Al 4V and rat osteoblast culture system can be used to evaluate the osteocompatibility of substituted materials in vitro.