HBV感染者中HBV前S1蛋白检测分析

Value of Pre s1 protein in diagnosis of HBV infection

目的检测HBV前S1蛋白(PreS1-Ag)的水平,分析与HBV DNA复制的关系。方法用酶联免疫吸附分析法(ELISA)检测HBV感染者血清中PreS1-Ag和HBV血清标志物(HBsAg、HBsAb、HBeAg、HBeAb、HBcAb),同时用Real Time PCR检测血清中HBV DNA含量。结果 407例HBV感染者HBeAg(+)的大三阳模式135例血清中,HBV-DNA、PreS1-Ag阳性率分别为87.4%和81.5%,两者差异无统计学意义;在117例HBeAg(-)的小三阳模式血清中,HBV-DNA、PreS1-Ag阳性检出率分别为74.4%和69.2%,两者差异无统计学意义。在266例HBV-DNA阳性患者血清中,PreS1-Ag阳性和HBeAg阳性检出率分别为74.4%、60.2%,两者差异具有统计学意义,P<0.01。结论 PreS1-Ag与HBV-DNA检测结果一致性较好,较HBeAg能更灵敏地反映体内HBV的复制状况,可与HBV-DNA互相补充,在临床诊断和治疗HBV感染中有重要意义。

Aim To investigate the value of Presl-Ag in diagnosis of HBV infection and its relationship between Pres1-Ag and HBV-DNA replication. Methods ELISA was used to detect serum levels of PreSl-Ag and HBV-M （HBsAg, HBsAb,HBeAg,HBeAb, HBcAb）,and real time PCR was employed to detect HBV-DNA contents in 407 cases with HBV infection. Results In 135 cases positive for HBsAg,HBeAg and HBcAb,the detection rates of HBV-DNA and Pres1-Ag were 87.4% and 81.5% respectively,without significant differences. In 117 cases positive for HBsAg, HBeAb and HBcAb, the detection rates of HBV-DNA,PreSl-Ag were 74.4% and 6912%, without showing dignificant differences. In 266 eases positive for HBV-DNA ,PreS1-Ag and HbeAg detection rates were 74.4% and 60.2% showing significant differences between them,P〈0.001. Conclusion The contents of PreS1-Ag is highly consistent with the concentration of HBV-DNA and the detection of PreS1-Ag can sensitively reflect the replication of HBV. The detection of HBV-DNA can be taken as a complement for diagnosis and treatment of HBV infection.