一株具免疫增强活性红树林真菌的筛选及鉴定

Screening and identification of a mangrove fungus possessing immunoenhancement activity

目的对海南红树林土壤层中分离和筛选具有免疫增强活性的真菌菌株进行鉴定,探讨红树林真菌在免疫调节方面的应用价值。方法通过MTT法对分离自红树林的真菌发酵上清进行检测,筛选具有免疫增强活性的菌株,结合形态学特征和rDNA ITS序列分析确定其种属分类地位。结果共分离到真菌325株,其中有12株具有促进人外周血淋巴细胞增殖作用,其中菌株PH0016有较强效果。菌落形态观察、光学显微观察、rDNA ITS序列测定鉴定菌株PH0016为淡紫色拟青霉(Paecilomyces lilacinus)。结论 PH0016是一株淡紫色拟青霉,其发酵上清具有一定的免疫增强活性,值得进一步研究其活性代谢产物。

Aim TO screen and identify fungi possessing immunoenhancement activity from mangrove soil collected from the sea area in Hainan and discuss the value of mangrove fungi in immunoloregulation. Methods The fungi possessing immunoenhancement activity were screened by using MTT method to measure the fermental supernatant. Identified and explore the taxonomic status of strains by means of morpholodical observations and analysis of the rDNA-ITS sequence. Results Totally 325 fungus strains were obtained from mangrove soil samples and 12 strains possessed immunoenhancement activity. The strain PH0016 could significantly stimulate lymphocyte proliferation. The strain was identified as Poecilomyoes ilacinus. Conclusions PH0016 is a Paecilomyces lilacinus,the fermental supernatant has immunoenhancement activiry,the active metabolites were worthy to be studied further.