益心口服液预处理对急性心肌缺血大鼠CEC、血浆ET、CGRP及心肌病理形态学的影响

Effects of preconditioning by yixin oral liquid on CEC,plasma ET & CGRP,myocardial pathomorphology of acute myocardial ischemia in rats

目的：研究益心口服液预处理对急性心肌缺血大鼠心血管的保护作用。方法：设假手术组、模型对照组、维拉帕米组、益心口服液组，建立急性心肌缺血模型。造模后２ｈ 时采血测血浆内皮素（ Ｅ Ｔ） 、降钙素基因相关肽（ Ｃ Ｇ Ｒ Ｐ） 、循环内皮细胞（ Ｃ Ｅ Ｃ） 计数，并进行心肌病理形态学观察。结果：益心口服液和维拉帕米均可降低急性心肌缺血大鼠血浆 Ｅ Ｔ（ Ｐ＜ ００５） 和 Ｃ Ｅ Ｃ（ Ｐ＜ ００１） 水平，减轻心肌病理形态学改变。维拉帕米组血浆 Ｃ Ｇ Ｒ Ｐ低于模型对照组（ Ｐ＜ ００５） 。益心口服液组和维拉帕米组间上述三指标无显著性差异。结论：益心口服液预处理可减轻心肌及血管内皮细胞损伤，调节急性心肌缺血时所发生的 Ｅ Ｔ、 Ｃ Ｇ Ｒ Ｐ分泌失衡。

OBJECTIVE:To investigate the protective effects of preconditioning by yixin oral liquid (YOL) on cardiovascular system of acute myocardial ischemia rats.METHODS:Acute myocardial ischemia models in control group,verapamil group and YOL were set up,meanwhile the sham group was built up.Blood would be collected for assaying endothelin (ET) and calcitonin gene-related peptide (CGRP) in plasma,circle endothelial cell (CEG),and myocardial pathomorphological examination would be preceded after the myocardial ischemia models being set up for two hours.RESULTS:Both YOL and verapamil could decrease not only the levels of plasma ET ( P <0 05) and the amounts of CEC ( P <0 01, P <0 05 respectively),but also ameliorate myocardial pathomorphological changes in acute myocardial ischemia rate.The contents of phasma CGRP in verapamil group were lower than those in model group ( P <0 05).Difference of the up-mentioned three indexes between YOL group and verapamil group appeared to be insignificant.CONCLUSION:Preconditioning by YOL might play an imporatant role in the modulation of plasma ET and CGRP as well as in the amelioration of myocardium and vascular endothelial cells in acute myocardial inchemia rats.