血小板衍生生长因子促进成骨细胞DNA合成的实验研究

The effects of plateletderived growth factor on rat osteoblastic DNA contents

目的探讨血小板衍生生长因子（ＰＤＧＦ）对成骨细胞ＤＮＡ合成和细胞周期变化的影响及其在骨折修复中的作用。方法从胎鼠颅骨分离、培养成骨细胞，应用流式细胞仪观察ＰＤＧＦ对成骨细胞ＤＮＡ合成及细胞周期的影响；通过透射电镜观察细胞的超微结构改变，了解ＰＤＧＦ在鼠成骨细胞分泌功能方面的作用。结果（１）ＰＤＧＦ对培养２４小时的成骨细胞Ｓ期的ＤＮＡ含量明显增高（１７７％）；（２）ＰＤＧＦ促使细胞内线粒体更丰富，粗面内质网增多。结论ＰＤＧＦ可通过刺激成骨细胞的ＤＮＡ合成，促进成骨细胞的分裂、增殖及细胞的合成分泌。

bjective To study the effects of plateletderived growth factor (PDGF) on the DNA content of the isolated osteoblastlike cells and the cellular period changes, and further study the effect of PDGF on bone fracture healing. Methods The cultured osteoblastlike cells in vitro were isolated from fetal rat calvaria and the effects of PDGF on cellular DNA contents were observed under flow cytometer. The changes of osteoblastic surface structure and ultrastructure were observed under electron microscope. Results PDGF could increase the DNA contents of cellular S period by promoting osteoblast from G0/G1 period into S period. The effects of PDGF on increasing cellular DNA contents were most significant at 24th culture hour (DNA content: 176% at the 24th hour, 90% at the 48th hour, 102% at the 72nd hour).PDGF could increase osteoblastic surface granules, reticular fibers and intracellular calcium salt crystals, and calcium granule mitochondria. ConclusionsBZPDGF could promote bone fracture healing by stimulating osteoblastic DNA synthesis and cell proliferation. The flow cytometer is a better instrument to analyze cell DNA content for a large number of cells.