高渗刺激诱导大鼠下丘脑室旁核和视上核神经激肽B受体阳性神经元表达Fos

NEUROKININ B RECEPTOR POSITIVE NEURONS IN THE PARAVENTRICULAR AND SUPRAOPTIC NUCLEI OF THE HYPOTHALAMUS EXPRESS Fos AFTER INTRA VENOUS INJECTION OF HYPERTONIC SALINE IN THE RAT

目的研究神经激肽Ｂ受体（ＮＫ３受体）与机体渗透压调节之间的可能关系。方法用双重免疫组织化学染色技术，高渗刺激采用静脉内注射１．５ｍｏｌ／ＬＮａＣｌ的方法，观察了下丘脑室旁核和视上核内ＮＫ３受体阳性神经元在高渗刺激下的Ｆｏｓ表达情况。结果高渗盐水可诱导下丘脑室旁核和视上核内大量神经元表达Ｆｏｓ。在室旁核，双标细胞约占ＮＫ３受体阳性神经元的８８％，约占Ｆｏｓ阳性细胞的７１％；在视上核的主部，双标细胞约占ＮＫ３受体阳性神经元的７４％，约占Ｆｏｓ阳性细胞的４３％。结论室旁核和视上核内ＮＫ３受体阳性神经元可能参与大鼠渗透压调节过程。

Objective\ In order to investigate possible relation of neurokinin B receptor(NK 3) with osmoregulation,expression of Fos in NK 3 receptor containing neurons in the paraventricular and supraoptic nuclei of the hypothalamus was investigated in the rat after hypertonic stimuli.Methods\ A double immunohistochemical staining method was used;hypertonic stimuli was done with intravenous injection of 1 5 mol/L NaCl.Results\ Hypertonic treatment induced Fos expression in many neurons of the paraventricular and supraoptic nuclei.In the paraventricular nucleus,about 88% of NK 3 receptor positive neurons exhibited Fos like immunoreactivity, which corresponded to about 71% of Fos positive neurons.In the principal part of the supraoptic nucleus,about 74% of NK 3 receptor positive neurons was Fos like immunoreactive,which corresponded to about 43% of Fos positive neurons.Conclusion\ NK 3 receptor containing neurons in the paraventricular and supraoptic nuclei might be involved in osmoregulation of the rat.