利用原位杂交分析几种cyclin和CDK基因在人乳腺癌中的异常表达

IN SITU HYBIDIZATION STUDY ON EXPRESSION OF SEVERAL cyclin AND CDK GENES IN HUMAN BREAST CARCINOMA

目的探讨细胞周期调控基因ｃｙｃｌｉｎ和ＣＤＫ的异常与乳腺癌发生的相关性。方法采集２０例乳腺癌外科手术样品，用原位杂交技术分析了ｃｙｃｌｉｎＤ１、Ｄ３、Ｅ、ＣＤＫ２和ＣＤＫ４ｍＲＮＡ的表达。结果在大多数乳腺癌样品中，这些基因有一种或几种出现高表达。ｃｙｃｌｉｎＤ１高表达的样品１７例（８５％），ｃｙｃｌｉｎＤ３有１２例（６０％），ｃｙ－ｃｌｉｎＥ有７例（３５％），ＣＤＫ２有６例（３０％），ＣＫＤ４有１６例（８０％）。ｃｙｃｌｉｎＤ１和ＣＤＫ４显色最强，其次是ｃｙｃｌｉｎＤ３，ｃｙ－ｃｌｉｎＥ和ＣＤＫ２显色较弱，正常乳腺组织为阴性显色。结论推测ｃｙｃｌｉｎ和ＣＤＫ基因，特别是ｃｙｃｌｉｎＤ１和ＣＤＫ４的异常表达与乳腺癌的发生有密切关系。

Objective\ Study on the relationship between dysregulated expression of cyclin and CDK genes and tumorigenecity of human breast carcinoma.Methods the expression of cyclin D1,D3,E,CDK2 and CDK4 mRNA of 20 human breast cancer specimens was analyzed.Resuts one or more than one of these genes overexpressed in the majority of the cancer specimens,namely cyclin D1 overexpressed in 17 cancer specimens(85%);cyclin D3 in 12(60%);cyclin E in 7(35%);CDK2 in 6 (30%);CDK4 in 16(80%).The staining intensity of cyclin D1 and CDK4 was strong,cyclin D3 was moderate,cyclin E and CDK2 was weak,the normal breast tissue was negative. Conclusions these data implicate that dysregulated expression of several cyclin and /or CDK genes,particularly cyclin D1 and CDK4,might act as potential factors in the pathogenesis of breast cancer.