孤儿肽在自发性高血压大鼠和家兔心血管系统的分布

CARDIOVASCULAR DISTRIBUTION OF ORPHANIN FQ IN SPONTANEOUSLY HYPERTENSIVE RAT AND RABBIT

目的研究孤儿肽（ｏｒｐｈａｎｉｎＦＱ，ＯＦＱ）在自发性高血压大鼠和家兔心血管系统的分布。方法采用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）法和免疫组织化学方法。结果ＲＴ－ＰＣＲ的结果显示，ＯＦＱ的前体ｍＲＮＡ在自发性高血压大鼠的主动脉、肺动脉、肾动静脉均有较高的表达，其表达水平与脑组织表达水平相似，心房组织也有弱的表达，但在心室组织未见ＯＦＱ前体ｍＲＮＡ的表达。免疫组织化学结果显示，心肌细胞ＯＦＱ免疫反应阴性，心房内膜的内皮细胞和主动脉、肾动静脉的内皮细胞及血管平滑肌细胞均含有ＯＦＱ免疫反应阳性颗粒，此外在肾小球也观察到了ＯＦＱ反应阳性细胞。结论ＯＦＱ可能是对心血管系统和肾脏功能有一定调节作用的调节肽，但其具体的功能及调节机制尚待进一步研究。

Objective\ To investigate the cardiovascular distribution of orphanin FQ (OFQ) and OFQ precursor mRNA in spontaneously hypertensive rat(SHR) and rabbit. Methods\ Reverse transcription polymerase chain reacrtion(RT PCR), and immunohistochemical method were used. Results\ The expression of OFQ precursor mRNA was detected from aorta, pulmonary artery, renal artery and vein of rat at a high level comparable with the amounts of brain, and a weak expression signal could also be observed in the atrium. The positive immunoreactive OFQ was detected in the tissues of aorta, atrium and renal of rabbit, as well as in smooth cells, endothelium and glomerulus. Conclusion\ These observations suggest that orphanin FQ might play a role in regulating the function of cardiovascular system and kidney, but the exact underlying mechanism needs further studying.