家系虚寒证与非家系虚寒证基因表达谱比较研究

Comparison of gene expression between family and non-family yang-deficiency syndrome

目的:探讨家系虚寒证与非家系虚寒证在宏观证候及微观基因表达的异同。方法:利用Array Tools和FatiGO等生物信息学软件挖掘基因表达谱数据,获得差异基因、差异功能类及差异分子通路。结果:家系虚寒证和非家系虚寒证在宏观证候和临床表现上没有显著差别,但基因表达水平上显著不同,家系虚寒证和非家系虚寒证差异表达的基因分别有136条和138条,其中只有2条相同,分别为NM003370、NM005252;家系虚寒证组差异功能分布相对集中,主要和能量代谢密切相关,而非家系虚寒证组差异功能分布比较广泛;家系虚寒证的差异通路有3条,而非家系虚寒证组差异通路有8条,二者没有相同的差异通路。结论:家系虚寒证和非家系虚寒证差异表达的基因种类、在染色体上的分布、影响的功能及通路都不相同。对家系虚寒证的基因表达谱分析只能适用于该家系病因分析,对非家系虚寒证的发生机制还需相关的实验研究。但可借鉴研究家系虚寒证基因表达谱的方法。

Objective： To explore the similarities and differences of family and non-family yang-deficiency syndrome in the macro syndrome and micro gene expression.Methods： In order to obtain the different genes,functional classes and molecular pathways,the various data mining methods of bioinformatics in gene expression profile,such as Array Tools and FatiGO were used.Results： The family and non-family yang-deficiency evidence at the macro syndrome and clinical performance was no significant difference,but level of the gene expression was significantly different.Differentially expressed genes of the family and non-family deficiency were respectively 136 and 138,of which only two the same,namely,NM003370,NM005252.Relating to differences function distributed more widely and had eight different channels in non-family yang-deficiency,the function distribution of family yang-deficiency was relative concentration,closely related to energy metabolism,and only had three different channels.Conclusion： The differentially expressed genes between the family and non-family yang-deficiency cards were different effects in the species,the distribution in chromosomes and functions,and played a role through different pathways.The evidence on the family yang-deficiency analysis of gene expression can only be applied to the family cause,the pathogenesis related to yang-deficiency syndrome of non-family need experimental studies.But can learn the method from family yang-deficiency.