红参超微饮片的指纹图谱研究

Studies on HPLC fingerprint of ultramicro Panax Ginseng rubra

目的:采用HPLC法对不同来源的红参超微饮片进行指纹图谱研究,为红参超微饮片质量控制提供依据。方法:色谱柱:Inertsil WondaSil C18(4.6mm×200mm,5μm)键合硅胶色谱柱;流动相:乙腈-水(梯度洗脱);流速:1.0mL/min,检测波长:203nm;柱温:35℃。结果:以11个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD均小于2%,10批样品相似度>0.9。结论:此方法精密度、稳定性和重复性良好,有助于红参超微饮片的质量控制。

Objective： The fingerprint chromatograms were established for quality evaluation of ultramicro Panax Ginseng rubra from different sources by HPLC.Methods： The analysis was performed on a Inertsil WondaSil C18 column（4.6mm×200mm,5μm） with acetonitrile-water as mobile phase in a gradient mode at a flow rate of 1.0mL/min,and at a column temperature of 35℃.The detection of wavelength was at 203nm.Results： 11 peaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 2% in the precision and repeated test.The similarity of 10 batches of samples was no less 0.9.Conclusion： The method was reliable and can be helpful on the quality control of ultramicro Panax Ginseng rubra