小承气汤中橙皮苷和肉桂酸的含量测定

Determination of the contents of hesperidin and cinnamic acid in Xiaochengqi decoction by HPLC

目的:建立小承气汤中橙皮苷和肉桂酸的HPLC同时测定方法,以便进行质量控制。方法:采用Apollo C18柱(250mm×4.6mm,5μm),流动相为乙腈和0.011%冰乙酸水溶液,梯度洗脱,时间40min,流速1mL/min,检测波长为283nm。结果:橙皮苷和肉桂酸的线性范围分别为0.20-0.80μg和0.05-0.33μg,精密度为0.24%和0.16%,平均回收率分别为96.3%和101.9%。在6批样品中,橙皮苷和肉桂酸的含量差别比较大。结论:文章建立的方法简便、灵敏、准确,可用于小承气汤的质量控制。

Object： To establish a RP-HPLC method for determination of hesperidin and cinnamic acid in Xiaochengqi decoction for the quality control of Xiaochengqi decoction.Methods：Apollo C18 column（250mm×4.6mm,5μm） was used with the mobile phase of acetonitrile and 0.011% acetic acid aqueous solution in gradient mode.The flow rate was 1.0mL/min,and the detection wavelength was set at 283nm.Results：The calibration curve was linear in the range of 0.20-0.80μg（r=0.9993） for the hesperidin and 0.05-0.33μg（r=0.9992） for the cinnamic acid.The inner-day RSD was 0.24% and 0.16%,and the average recovery was 96.3% and 101.9%,respectively.The content changes of the components are significant between batches.Conclusion：This method is accurate,simple and sensitive,and can be used for the quality control of Xiaochengqi decoction.