摘要
目的观察高能震波（ＨＥＳＷ）对骨痂中骨形成蛋白（ＢＭＰ）表达的影响，探讨其促进骨折愈合的机理。方法手术造成家兔双侧胫骨中段标准骨折模型，对一侧胫骨骨折部施以ＨＥＳＷ，另一侧为对照组。术后每周进行Ｘ线及组织学检查，并取骨痂组织切片用ＢＭＰ抗体进行免疫组化染色（ＳＡＢＣ法）。结果实验组骨痂中ＢＭＰ表达强度在第２～４周时明显大于对照组（Ｐ＜００１），且Ｘ线摄片及组织学观察均显示实验组骨折愈合时间早于对照组。结论ＨＥＳＷ能够增加骨痂中ＢＭＰ的表达，加强诱导成骨，从而促进骨折愈合。其可望成为一种非手术治疗骨折愈合障碍的新方法。
Objective To detect the effects of high energy shock waves (HESW) on
fracture healing and to explore its mechanisms Methods Standardized fracture models were
made in bilateral tibial diaphyses in 30 rabbits Seven days after fracture, one side of the model
was treated with HESW (145 kV, 500 shock waves), the another side served as controls The
X-ray films and histological sections were made for the evaluation of effects on fracture healing
At the different stages, rabbits fracture callus and BMP antibody were adopted for
immunohistochemical staining (SABC method) Results The fracture healing found in
experimental group were earlier than that of the control group The intensity of the positive
staining of experimental group was more than that of the control group (P< 001) Conclusion The
result suggests that HESW can increase the expression of BMP in fracture callus, and can
stimulate callus formation and accelerate fracture healing
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1999年第5期295-298,I001,共5页
Chinese Journal of Orthopaedics
