摘要
比较了凝胶电泳法检测质粒 Ｄ Ｎ Ａ 时不同激发波长对 Ｄ Ｎ Ａ Ｅ Ｂ荧光强度的影响，发现短波长激发光可增加 Ｄ Ｎ Ａ 的探测灵敏度。采用 ２６０ｎｍ 作为激发光时可探测到少至 ０７ｎｇ 的线性 Ｄ Ｎ Ａ，且在很广的 Ｄ Ｎ Ａ 质量范围内， Ｄ Ｎ Ａ Ｅ Ｂ荧光强度与 Ｄ Ｎ Ａ 量成正比。以此改进方法检测电离辐射诱导的 Ｄ Ｎ Ａ 单、双链断裂，可得到与其它研究结果相一致的 Ｇ（ Ｓ Ｓ Ｂ）和 Ｇ（ Ｄ Ｓ
Influence of exciting wavelength on DNAEB fluorescence intensity was compared in detecting plasmid DNA of gel electrophoresis. It was found that short exciting wavelength enhanced the detecting sensitivity to DNA fractions. Using 260 nm as the exciting wavelength, as little as 0.7 ng linear DNA was measurable, and the fluorescence intensity of DNAEB complex proportioned to DNA quantity of a wide range. Furthermore, measuring irradiationinduced DNA strand breaks with this modified method, G(SSB) and G(DSB) consisted with other researches were obtained.
出处
《激光生物学报》
CAS
CSCD
1999年第3期238-238,F003,共2页
Acta Laser Biology Sinica
基金
国家自然科学基金
关键词
凝胶电泳
质粒DNA
链断裂
检测灵敏度
Gel electrophoresis
plasmid DNA
strand breaks
detecting sensitivity
