大鼠脑缺血区非特异性酯酶阳性细胞的分布和定量研究

A STUDY OF QUANTITATION AND DISTRIBUTION OF NONSPECIFIC ESTERASE POSITIVE CELLS IN ISCHEMIC BRAIN IN RATS

目的 探查非特异性酯酶阳性细胞的变化和与脑缺血的病理联系。 方法 用酶组织化学方法对４０只ＳＤ大鼠脑局部缺血／再灌流损伤区非特异性酯酶阳性细胞的分布和数量进行了观查。 结果 脑缺血区非特异性酯酶阳性细胞的数量增加发生于脑缺血１ｈ 再灌流２ｈ，随后逐渐增加，在脑缺血１ｈ 再灌流１６ｈ 达到高峰。阳性细胞的增加显示明显的时间依赖关系。阳性细胞主要位于小血管和微血管的壁内及其周围。在缺血侧基底节区非特异性酯酶阳性细胞比皮质缺血区多。 结论 非特异性酯酶阳性细胞对脑缺血刺激反应敏感，在缺血侧基底节区，其反应更为强烈，此因素可能是基底节区易发生中风的原因之一。

Objective\ To study the relationship of the profile and distribution of nonspecific esterase positive cells with cerebral ischemica.Metholds We explored the distribution and the number of nonspecific esterase positive cells in the ischemic brain of 40 SD (Sprague dawley) rats by using enzyme histochemical methods.Results The nonspecific esterase positive cells were mainly located in the walls of small vessels and microvasculatures and around them, the cells were increased in ischemic brain at 1 hour ischemia/2 hour reperfusion, and they peaked at 1 hour ischemia/16 hour reperfusion, and the nonspecific esterase positive cells in ischemic basal ganglia were more than that in ischemic cortex.Conclusion Cerebral ischemia induced the positive cell infiltration and basal ganglia may be more susceptible to ischemia than cortex.