人类组织相容性抗原-GmRNA表达与不明原因习惯性流产关系的初步研究

Preliminary Study on the Relationship between Human Histocompatibility Leukocyte Antigen G mRNA and Recurrent Spontaneous Abortion

目的 探讨人类组织相容性抗原Ｇ（ＨＬＡＧ） ｍＲＮＡ 的表达与不明原因习惯性流产（ＲＳＡ） 的关系。方法 用原位杂交的方法检测正常妊娠早期、晚期孕妇胎盘和ＲＳＡ患者经免疫治疗并成功分娩的胎盘母胎界面处的ＨＬＡＧｍＲＮＡ的表达。结果 正常妊娠早期、晚期孕妇胎盘母胎界面均有ＨＬＡＧｍＲＮＡ的阳性表达，阳性表达位于绒毛内（ 或） 外细胞滋养细胞，以及合体滋养细胞的胞浆内。ＲＳＡ患者免疫治疗后的胎盘均不表达ＨＬＡＧｍＲＮＡ，免疫治疗没有调节ＨＬＡＧ ｍＲＮＡ 表达的作用。结论 ＨＬＡＧ 可能与ＲＳＡ的发病原因没有直接的关系；与胎儿存活也无直接关系。

Objective To examine the expression of Human Histocompatibility Leukocyte Antigen G (HLA G) mRNA in placenta from cases of recurrent spontaneous abortion (RSA) and try to study the relationship between HLA G mRNA and RSA. Methods In situ hibridization was performed on maternal fetal interface sections of placenta in first trimester, third trimester of normal pregnancy and RSA women who were treated with immunotherapy and had term delivery. Results The positive signal of HLA G expression were showed in all placentas of nomal pregnant women within villus and cytoplasma of syncytiotrophoblast and cytotrophoblast. However, there is no any HLA G mRNA signals in placentas of RSA women after immunotherapy. Conclusions HLA G mRNA is not the main cause of RSA and there is no direct relations to fetal survival.