离体异位子宫内膜细胞中单核细胞趋化蛋白-1的测定

Determination of Monocyte Chemotactic Protein 1 in Cultured Endometriotic Cells

目的 探讨异位子宫内膜细胞产生单核细胞趋化蛋白１（ＭＣＰ１）的临床意义。方法 收集１５ 例子宫内膜异位症患者的异位内膜组织进行体外细胞培养，分别加入纯培养液（ 对照组） 、含白细胞介素１β（ＩＬ１β）２ ｎｇ／ｍｌ（ＩＬ１β诱导组）及肿瘤坏死因子α（ＴＮＦα）２０ ｍｇ／ｍｌ（ＴＮＦα诱导组）的培养液培养４ 小时，应用斑点杂交、免疫组织化学及夹心酶联免疫吸附试验检测各组异位细胞中ＭＣＰ１ｍＲＮＡ蛋白的表达水平及其培养上清中ＭＣＰ１ 的含量。结果 与对照组相比，异位内膜细胞经ＩＬ１β及ＴＮＦα作用后表达的ＭＣＰ１ ｍＲＮＡ和蛋白水平显著增加（ Ｐ＜ ０．０１ ，Ｐ＜０．０５）；培养上清中ＭＣＰ１ 的含量也显著增加（Ｐ＜０ ．０１ ，Ｐ＜ ０．０５）。结论 ＩＬ１β及ＴＮＦα可刺激异位内膜细胞中ＭＣＰ１ 的表达显著增加，这可能和子宫内膜异位症的病变发展有关。

Objective To investigate the clinical significance of monocyte chemotactic protein 1 (MCP 1) produced by endometriotic tissues. Methods Expressions of MCP 1 mRNA and MCP 1 protein were determined by dot blot analysis, immunohistochemical method [streptavidin biotin peroxidase complex (SABC)] and enzyme linked immunosorbent assay (ELISA) methods in cultured endometriotic cells with median (controls) or with interleukin 1β(IL 1β)2 ng/ml (IL 1β group), and tumor necrosis factor α(TNF α)20 mg/ml(TNF α group) respectively. The endometriotic tissues were sourced from 15 patients with endometriosis. Meanwhile, the MCP 1 protein content of cultured supernatent was also measured. Results After exposured to IL 1β or TNF α, the expression of MCP 1 mRNA and MEP 1 protein in the endometriotic cells were significantly higher than that in the control group ( P <0.01, P <0.05 respectively); so was the MCP 1 protien content of supernatent. Conclusions IL 1 β and TNF α can up regulate the expression of MCP 1 in endometriotic cells, which may be related to the development of emdometriosis.