人胃癌转移鼠模型的构建及移植瘤的阶段性研究

The construction of human gastric carcinoma metamouse model and the study on phases of transplantation tumor

目的：建立人胃癌的“转移鼠”模型，为研究胃癌转移打下基础。方法：将人胃低分化粘液腺癌 Ｍ Ｇ Ｃ８０３ 细胞悬液先在裸小鼠皮下接种成瘤，再取瘤组织块分别接种到裸鼠的皮下、胃壁上（ 转移鼠模型） ，分早（ ＜ ２０ 天） 、中（２０ ～４０ 天） 、晚（ ＞ ４０天）３ 个阶段观察移植瘤的生长及侵袭转移情况。结果：皮下组移植成瘤率为８２１ ％ （２３／２８） ，早、中阶段未见转移，晚期肺转移率５７１ ％ （４／７） 。胃壁组移植成瘤率为８６９ ％ （２０／２３） ，早期、中期未见转移，晚期肺、肝和胃周淋巴结转移率都为５００ ％（３／６） ，并广泛侵犯其他器官。结论：“转移鼠”模型能模拟临床胃癌病人的发展过程，转移是一个晚期事件。

Purpose To construct metamouse model of human gastric carcinoma, which will provide us with a basis to study gastric carcinoma metastasis. Methods The cell suspensions of human gastric poor differentiated mucinous adenocarcinoma, MGC 803, were inoculated into nude mice subcutis. Then, the histologically intact xenograft tissues were transplanted to subcutic of nude mice, stomach wall (so called metamouse), respectively. The biological characteristics of the ecotopic and orthotopic xenografts in three periods early(<20 d), middle(20～40 d) and advanced(>40 d)) were observed. Results The take rate of the subcutaneous transplantation group was 82 1%(23/28). Metastasis did not happen in the early and middle phases but the incidence of pulmonary metastasis was 57 1%(4/7) in the advanced period. In contrast, the take rate of the stomach wall transplantation group amount to 86 9%(20/23), also, metastasis was observed only in the advanced period. Lung, liver and stomach regional lymph node metastases were all of the same incidence, 50 0%(3/6), and there existed extensive spread to other organs. Conclusion Metamouse model closely correlates with the course of the tumor in the clinical patients and tumor metastasis is a late incident.