腰椎间盘蛋白多糖核心蛋白的基因表达

Expression of Proteoglycan Coreprotein Gene in Human Lumbar Discs

目的 探讨在椎间盘中核心蛋白ｍＲＮＡ 表达的增龄性变化。方法 通过ＲＴ－ ＰＣＲ制备核心蛋白特异性的ｃＤＮＡ 探针，应用原位杂交的方法，分别对胎儿和成人椎间盘标本进行核心蛋白ｍ ＲＮＡ表达的定位观察。结果 在胎儿椎间盘标本中，髓核内呈现高表达，纤维环和髓核交界处表达减弱，纤维环处未见阳性表达信号。在成人标本中，髓核和纤维环均未见明显的阳性表达。结论 在胎儿椎间盘核心蛋白主要在髓核高表达，从髓核到纤维环其表达依次降低。

Objective\ To observe the expression of coreprotein gene in human lumbar discs in foetus and adult. Methods\ The coreprotein cDNA probe was prepared by RT-PCR. The mRNA level and distribution of coreprotein was observed in fetal and adult lumbar discs, using in situ hybridization method. Results\ 1) The highest expression lies in the lumbar nucleus pulposus of the fetal discs, less higher expression in the interior of the annulus fibrosus and the region between nucleus and annulus fibroses. 2) In adult disc tissue, few positive signals were shown. Low gene expression level of coreprotein in the subcultured disc cells was expressed. Conclusion\ The higher expression of coreprotein in fetal discs is chiefly located in the nucleus pulposus.