PMMA骨水泥颗粒对大鼠巨噬细胞SOCS1及SOCS3基因表达的影响

The effect of PMMA bone cement particles on the expression of SOCS1 and SOCS3 genes in rat macrophages

目的观察PMMA骨水泥颗粒对体外培养的巨噬细胞SOCS1及SOCS3基因表达的影响。方法大鼠腹腔灌洗液筛选法培养巨噬细胞,CD68单抗免疫细胞化学法鉴定细胞纯度和活性,实时荧光定量PCR(Real-time PCR)方法检测巨噬细胞SOCS1及SOCS3基因的表达量,分别观察不同浓度PMMA骨水泥颗粒及一定浓度的骨水泥颗粒作用不同时间对巨噬细胞SOCS1及SOCS3基因表达的影响。结果 PMMA骨水泥颗粒促进了体外培养的巨噬细胞SOCS1及SOCS3基因的表达,并表现出一定的浓度及时间相关性;颗粒浓度在0.25 mg/mL时SOCS1及SOCS3基因表达量明显升高(P<0.05);当颗粒作用时间在24 h左右时SOCS1及SOCS3基因表达升高最显著(P<0.05)。结论 PMMA骨水泥颗粒能够促进巨噬细胞SOCS1及SOCS3基因的表达。

Objective To observe the effect of PMMA bone cement particles on the expressions of SOCS1 and SOCS3 genes by rat macrophages in vitro. Methods Macrophages were obtained from the peritoneal of rats and cultured. Cell purity was identified with immunocytochemistry using monoclonal antibody against CD68. Real-time PCR method was used to detect the expression levels of SOCS1 and SOCS3 genes. The effects of PMMA bone cement particles on the expression of SOCS1 and SOCS3 genes were observed at different concentrations and time. Results PMMA bone cement particles increased the expression of SOCS1 and SOCS3 in cultured macrophages, with a certain time- and dose-dependent fashion. The expression of SOCS1 and SOCS3 increased significantly at a concentration of 0.25 mg/ml of PMMA （ P 〈 0.05 ） , and elevated the most significantly in cultures at 24h （P 〈 0.05）. Conclusion PMMA bone cement particles can stimulate the expression of SOCS1 and SOCS3 in macrophages.