采用酶联免疫捕获法和荧光酶联免疫吸附法检测过敏原IgE抗体的比较分析

Capture ELISA and FEIA assay for allergen-specific IgE antibody:a comparative analysis

目的:对酶联免疫捕获法的ALLERG-O-LIQ系统和荧光酶联免疫吸附法的ImmunoCAP系统检测过敏原特异性IgE抗体的一致性进行对比观察。方法:研究对象为我院2008～2010年就诊于变态反应科、儿科的病例,采用2种不同的酶联免疫吸附法检测屋尘螨(D1)、粉尘螨(D2)、狗毛屑(E5)、德国小蠊(I6)、鸡蛋(F1)、牛奶(F2)、花生(F13)、蟹(F23)、虾(F24),共9种过敏原特异性IgE抗体检测,将2种检测结果进行一致性检验和线性回归分析。结果:共进行了520项次过敏原特异性IgE检测,两种系统对吸入性过敏原特异性IgE抗体定量检测结果一致性良好(P<0.05),特异性IgE结果:屋尘螨>粉尘螨>德国小蠊>狗毛屑;而对食物过敏SIgE抗体检测结果的相关度较吸入过敏原低,特异性IgE结果:蟹>虾>花生>鸡蛋>牛奶。结论:ALLERG-O-LIQ系统和ImmunoCAP系统都属于新一代易操作的过敏原检测系统。2种检测方法一致性良好,结果偏离度较小,呈线性回归分布。因此,对于评估血清中的特异性IgE来说,ALLERG-O-LIQ系统是一个可靠的、经济的临床诊断系统。其检测的结果与ImmunoCAP系统的检测结果比较相符合。

Objective：Enzymes immune to the capture of the law ALLERG-O-LIQ system and enzyme immune to the fluorescence detection method ImmunoCAP system allergic IgE the specifisity antibodies of observation.in contrast.Methods：The object of study is for the patient＇s cases 2008-2010 in allergies and pediatrics departments.Adopt two different enzymes immune to the original specificity IgE antibodies detect.There are 9 kinds of allergic,consist of D.pteronyssinus（D1）,Dermatophagus farinae（D2）,Dog dander（E3）,Blattella Germanica（I6）Egg（F1）,Milk（F2）,Peanuts（F13）,Crub（F23）,Shrimp（F24）.And the two inspection results in linear regression tests and consistency.Results：There are 520 times allergic to the original specificity IgE detect.Two systems for inhaling of the antibodies specificity IgE an allergy test results of consistency good（P0.05）,specificity IgE result：D.pteronyssinus Dermatophagus farinae Blattella Germanica Dog dander,but with food allergy test results of the antibodies sige relevance more into allergic to the low,Specificity IgE Result：CrubShrimp PeanutsEggMilk.Conclusion：ALLERG-O-LIQ syaterm and ImmunoCAP systerm both belong to the new generation is allergic to the operation of the detection system,Two detection methods consistent,from a relatively small,a linear regression distribution,So,with the serum Specificity IgE systerm of ALLERG-O-LIQ is a reliable,economic and clinical diagnosis.The test results and ImmunoCAP system compatible test results.