碳纤维复合材料磨损颗粒的生物学反应观察

Observation of Bioeffects of Wear Debris of Carbon Fiber Composite Materials

目的 观察聚砜(PSF)及其碳纤维复合材料(CPSF)的生物相容性。方法 本实验用聚砜及其碳纤维复合材料磨损颗粒在体外对巨噬细胞株ANA- 1 吞噬功能和释放肿瘤坏死因子(TNF)、白细胞介素6(IL- 6)功能的影响进行研究,同时观察其关节内注射后对滑膜细胞的影响,并与传统的7 种人工关节材料比较。结果 颗粒被吞噬的情况与其大小关系密切,< 10 μm 的颗粒易被吞噬,> 20 μm 则多发生细胞包绕颗粒的情况。各实验组TNF和IL-6 的释放都有增高,但实验组之间差异无显著性意义。颗粒注入关节后发现滑膜细胞的吞噬功能增强,A 型细胞多见。结论 聚砜及其碳纤维复合材料磨损颗粒的生物相容性良好,力学性能优良。

Objective To observe the biocompatibility of wear debris of polysulfone (PSF) and carbon fiber reinforced polysulfone (CPSF) by compring the in vitro influence on phagocyte and production of tumor necrosis factor (TNF), interleukin-6 (IL-6) of macrophage ANA-1 Methods 1)Preparation of debris and soliquoids of PSF, carbon fiber , CPSF, ultrahigh molecule weight polyethylene (PE), polymethylmethacrylate (PMMA), alumina (Al 2O 3), zirconia (ZrO 2),cobalt chromium molybdenum alloy (Co Cr Mo) and hydroxylapatite (HA) 2)Effects of wear debris on the functions of ANA-1 macrophage and its IL-6 and TNF production were compared 3)Effects of wear debris on the synovium of rabbits were observed Results The results indicated that there was a close relationship between the situation of phagocyte and size of debris The debris were phagocyted when the size was smaller than 20 μm The amount of TNF and IL-6 production was increased in the experimental groups, but there was no significant difference Conclusion The results of the experiment shows that PSF and CPSF have good biocompatibility and mechanical property, it is a promising biological composite material