金黄色葡萄球菌L型感染对小鼠垂直传播及DNA原位杂交的研究

A STUDY ON VERICAL TRANSMISSION STAPHYLCOCCUS AUREUS L-FORMS INFECTION IN MICE WITH NUCLEIC ACID HYBRIDIZATION IN SITU

本文用金黄色葡萄球菌及其稳定 Ｌ型， 经多种方式感染雌性昆明种小鼠， 发现雌鼠妊娠率、每胎分娩的子鼠数及子鼠体重均低于对照组， 差异有显著性 （ Ｐ＜ ００１ ， Ｐ＜ ００１ ， Ｐ＜ ００５） 。对感染鼠子宫内膜、胎盘及子鼠肝、肺等组织作免疫组化及金葡菌 Ｌ型地高辛核酸探针原位杂交， 结果发现金葡菌 Ｌ 型感染雌鼠子宫内膜、胎盘及子鼠肝、肺中金葡菌 Ｌ型免疫组化阳性率分别为６０５ ％ 、９３３ ％ 及６０ ％ ： 金葡萄 Ｌ 型 Ｄ Ｎ Ａ 探针原位杂交后在感染鼠子宫内膜、胎盘绒毛及子鼠肺组织中均发现有金葡菌 Ｄ Ｎ Ａ 表达， 阳性率分别为２０８ ％ 、３３３ ％ 、１６６ ％ ， 均高于对照组。提示 Ｌ 型金黄色葡萄球菌可通过垂直传播感染方式导致２ 代动物先天性感染， 这可能是引起妊娠异常结局的重要因素之一。

Vertical transmission were observed in mice after intravenous and ittravaginal infection with Staphylcoccus aureus and its stable L-forms.It was found that the rate of pregnancy and weight of new born mice in the infected groups were lower than that in the uninfected control group (P<0 01,P<0 01,P<0 05).In examination of the infected group,the S aurus L-forms were positive in endometrium and placenta of the mother mice and in liver and lung of the fetus.They were 60 5%,93 3% and 60% with immunohistochemical technique Nucleic acid hybridization in situ,positive rate was 20 8%,33 3% and 16 6% respectively.The positive rate in the infected group were higher than the control group.This indicates that the bacterial L-forms may pass through the placental barrier and transmit vertically to the fetal mice. \ \