水通道蛋白1和3在小鼠前列腺组织的表达及意义

Expressions of aquaporins 1 and 3 in the mouse prostate and their significance

目的：揭示水通道蛋白（AQP）在小鼠前列腺组织的表达及意义,为深入研究前列腺正常及某些病理状态下AQPs表达调控及生理功能奠定基础。方法：取小鼠前列腺组织,采用RT-PCR方法检测AQP 0~4mRNA在前列腺组织的表达,同时应用Western印迹和免疫组化研究AQP1及AQP3在前列腺组织的定位表达。结果：RT-PCR显示前列腺组织中有AQP1、3 mRNA表达,而未见AQP0、2、4 mRNA的表达。Western印迹结果显示在正常前列腺组织中表达相对分子质量为28 000的AQP1蛋白,以及相对分子质量分别为35 000和27 000的糖基化和非糖基化AQP3蛋白。免疫荧光和免疫组化结果表明小鼠前列腺近管腔处分泌细胞的胞内囊泡及细胞膜均可见较强的AQP1蛋白表达;而在前列腺基质上皮细胞有AQP3的表达。结论：AQP1和AQP3基因在前列腺分泌上皮细胞表达,提示AQP1和AQP3可能通过促进前列腺上皮细胞对水的渗透,在前列腺液分泌过程中发挥重要的生理作用。

Objective： To investigate the expressions of aquaporins（AQPs） in the mouse prostatic tissue and their significance,and to provide some evidence for a deeper insight into the physiological function and regulation of AQP expressions in normal and diseased prostatic tissues.Methods： The mRNA expressions of AQP0-4 in the mouse prostatic tissue were determined by RT-PCR,and the expressions and localizations of AQP1 and AQP3 proteins were characterized by Western blot and immunohistochemistry.Results： RT-PCR exhibited the mRNA expressions of AQP1 and AQP3,but not those of AQP0,AQP2 and AQP4 in the prostate tissue,while Western blot showed the expression of the AQP1 protein with the relative molecular mass（RMM） of 28 000 and those of the glycosylated and non-glycosylated AQP3 proteins with the RMM of 35 000 and 27 000,respectively.Immunohistochemistry indicated the strong expression of AQP1 in the cyst and plasma membrane of the secretary cells and that of AQP3 in the stroma cells of the prostate.Conclusion： The AQP1 and AQP3 genes were expressed in the secretary epithelia of the mouse prostate tissue,which suggests that AQP1 and AQP3 may play an important role in the secretion of prostatic fluid.