期刊文献+

血清视黄醇结合蛋白4对胰岛素抵抗大鼠骨骼肌组织磷脂酰肌醇3激酶的影响 被引量:4

Effects of retinol binding protein-4 on PI3K in somatic muscle tissue of insulin resistant rats
下载PDF
导出
摘要 目的研究视黄醇结合蛋白4(RBP4)对胰岛素抵抗大鼠骨骼肌组织磷脂酰肌醇3激酶(PI3K)的影响。方法雄性Wistar大鼠以高脂高糖饲料喂养诱发胰岛素抵抗模型,造模成功后随机分成正常对照(NC)、胰岛素抵抗(IR)和吡格列酮治疗(IR+PIO)三组。喂养8周后处死动物,取血清测血糖(FPG)、胰岛素(FINS)、甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL),计算胰岛素敏感指数(ISI),ELISA法检测血清RBP4水平,免疫组化方法检测骨骼肌组织中磷脂酰肌醇3激酶(PI3K)表达水平。结果 (1)IR组大鼠RBP4、FINS、LDL、腹部脂肪含量与体重比值高于NC组和IR+PIO组(P<0.01),IR组ISI、HDL低于其他两组(P<0.01),IR+PIO组FBG、TC、TG低于IR组(P<0.01);(2)IR组骨骼肌组织PI3K的表达低于NC组和IR+PIO组(P<0.01);(3)血清RBP4与骨骼肌组织PI3K(r=-0.577,P<0.01)、HDL(r=-0.550,P<0.01)、ISI(r=-0.457,P<0.01)呈负相关,与FINS(r=0.604,P<0.01)、腹部脂肪含量与体重比值(r=0.472,P<0.05)、LDL(r=0.337,P<0.05)呈正相关。结论胰岛素抵抗大鼠血清RBP4升高,吡格列酮可降低其水平。RBP4与大鼠骨骼肌组织PI3K水平负相关,提示其降低胰岛素的敏感性可能与阻碍胰岛素信号转导有关。 Objective To investigate effects of Retinol binding Protein-4(RBP4) on Phosphatidylinositol 3 Kinase(PI3K) in somatic muscle tissue of Insulin resistant rats.Methods We seted up insulin resistant rat model on a high fat diet.Then the rats were divided into normal control group(NC),insulin resistant group(IR) and IR model plus pioglitone therapy group(IR+PIO)at random.After 8 weeks,their blood was extracted for measuring the levels of glucose and serum RBP4,insulin,HDL,LDL,TG,TC.Immunohistochemistry was applied to detect the expression levels of PI3K in Somatic Muscle tissue.Results(1)The RBP4,FINS,LDL,ratio of visceral fat content over body weight,were significantly increased in RI group compared with control or IR+PIO(P0.01);ISI,HDL were significantly lower in IR group compared with control or IR+PIO(P0.01);FBG,TC,TG in IR+PIO group were significantly lower than those in IR group.(2)The expression levels of PI3K of IR group were significantly lower than those of both control group and IR+PIO group in somatic muscle tissue(P0.01);(3)RBP4 was directly coorelated with PI3K in somatic muscle tissue(r=-0.577,P0.01),FINS(r=0.604,P0.01),ratio of visceral fat content over body weight(r=0.472,P0.05),LDL(r=0.337,P0.05),HDL(r=-0.550,P0.01)and ISI(r=-0.457,P0.01).Conclusion The level of RBP4 were increased in IR group and pioglitone could decrease its level.RBP4 was directly coorelated with the levels of PI3K in somatic muscle tissue of IR rats,the mechanisms degrading insulin sensibility might be closely related with the interfere of insulin signal transduction in target tissues.
出处 《中外医学研究》 2011年第18期11-14,共4页 CHINESE AND FOREIGN MEDICAL RESEARCH
关键词 视黄醇结合蛋白4 胰岛素抵抗 磷脂酰肌醇3激酶 吡格列酮 Retinol binding protein-4 Insulin resistant Phosphatidylinositol 3 kinase Pioglitone
  • 相关文献

参考文献9

  • 1Yang Q, Graham TE, Mody N, et al. Serum retinol binding protein contributes to insulin resistance in obesity and type 2 diabetes. Nature,2005,436:356 - 362.
  • 2Marone R, Cmiljanovic V, Giese B, et al. Targeting phosphoinosit- ide 3 - kinase - Moving towards therapy. Biochim Biophys Acta, 2007 : 12.
  • 3Hotamisligli GS, Budavari A, Murray D, et al. Reduced tyrosine Kinase activity of the insulin receptor in obesity diabetes. J Clin Invest, 1994,94:543.
  • 4邹大进,吴鸿.腹型肥胖致胰岛素抵抗的机制及治疗展望[J].中国糖尿病杂志,2006,14(4):309-312. 被引量:39
  • 5Gavi S, Stuart LM, Kelly P, et al. Retinol - binding protein 4 is associated with insulin resistance and body fat distribution in non- obese subjects without type 2 diabetes. J Clin Endocrinol Metab, 2007,92 (5) : 1886 - 1890.
  • 6吴海娅,贾伟平,魏丽,陆俊茜,包玉倩,项坤三.肥胖及2型糖尿病患者血清视黄醇结合蛋白4水平的变化及其临床意义[J].中华内分泌代谢杂志,2006,22(3):290-293. 被引量:95
  • 7Von Eynatten M, Lepper PM, Liu D, et al. Retinol - binding pro- tein 4 is associated with components of the metabolic syndrome, but not with insulin resistance, in men with type 2 diabetes or cor- onary artery disease. Diabetologia,2007,50 (9) : 1930 - 1937.
  • 8C.H. Lee, P. Olson and R. M. Evans. Minireview:lipid metabo- lism, metabolic diseases, and peroxisome proliferator - activated receptors. Endocrinology,2003,144 (6) :2201 - 2207.
  • 9Fernandez AZ. PPARs as targets for the modulation of cardiovas- cular risk factors associated with the metabolic syndrome. Curr Opin Investig Dnigs ,2004,5 (9) :936 - 940.

二级参考文献24

共引文献130

同被引文献69

  • 1王雪松,郑芸,方积年.积雪草中降血糖多糖的研究[J].中国药学杂志,2005,40(22):1697-1700. 被引量:12
  • 2中国心脏调查组,胡大一,潘长玉.中国住院冠心病患者糖代谢异常研究——中国心脏调查[J].中华内分泌代谢杂志,2006,22(1):7-10. 被引量:470
  • 3吴海娅,贾伟平,魏丽,陆俊茜,包玉倩,项坤三.肥胖及2型糖尿病患者血清视黄醇结合蛋白4水平的变化及其临床意义[J].中华内分泌代谢杂志,2006,22(3):290-293. 被引量:95
  • 4聂娟,丁启龙.高葡萄糖和高胰岛素对葡萄糖转运子4影响的研究[J].医学综述,2007,13(11):826-828. 被引量:3
  • 5Nyholm B,Nielsen MF,Kristensen K,et al.Evidence of increased visceral obesity and reduced physical fitness in healthy insulin-resistant first-degree relatives of type 2 diabetic patients.Eur J Endocrinol,2004,150(2):207.
  • 6Bray GA.Health hazards of obesity.Endocrinal Metb Clin Nor Am,1996,25:907.
  • 7Das A,Mcguire PG,Eriqat C,et al.Human diabetic neovascular membrance contain high levels of urokinase and metallo-proteinase enzymes.Invet Ophthalmol Vis Sci,1999,40:809.
  • 8Lahdenera S,Sane T,Vuorinen-Markkola H,et al.LDL partical size in moderately hypertriglyceridemic subjects:relation to insulin resistance and diabetes.Atherosclerosis,1995,113:227.
  • 9Soumyanath A,Zhong YP,Gold SA,et al.Centella asiatica accelerates merve regeneration upon oral administration and contains multiple active frations increasing neurite elongation in-vitro.J Pharmacol,2005,57(9):1221.
  • 10Yoshida M,Fuchigami M,Nagao T,et al.Antiproliferative constituents from Umbelliferae plants Ⅶ.Active triterpenes and rosmatinic acid from Centella asiatica.Biol Pharm Bull,2005,28(1):173.

引证文献4

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部