期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人肺腺癌肿瘤细胞免疫表型CDl33、CD34、CD44的实验研究 被引量：3

Experimental research of immunophenotype CD133, CD34, CD44 in human lung adenocarcinoma c
原文传递
导出
摘要 目的拟验证CDl33、CD34、CD44作为人肺腺癌肿瘤干细胞表面标记物的合理性。方法采集新鲜肺腺癌组织标本，利用两种体外培养方法扩增出贴壁细胞和悬浮细胞球两种肿瘤细胞，采用免疫荧光检测比较CDl33、CD34和CD44在两种培养细胞中表达的差异。结果悬浮球肿瘤细胞培养较贴壁肿瘤细胞生长速度慢、维持时间长且成功率高（72．5％VS47．5％，P〈0．05）。CDl33、CD34和CD44在悬浮细胞球中表达率和表达量明显高于贴壁肿瘤细胞（68．97％，82．76％，93．10％VS5．26％，15．79％，5．26％，P〈0．01）。结论CDl33、CD34和CD44可能作为分离人肺腺癌肿瘤干细胞的表面蛋白表标记组合。 Objective To validate the possibility of CD133 CD34 CD44 be served as biomarkers in cancer stem cell of human lung adenocarcinoma. Methods Two kinds of culturing methods were performed to generate adhesive tumor cells and floating aggregates, and the differences of expression of CD133 CD34 CD44 between 2 kinds of cultured cells were observed by immunofluorescence. Results Floating aggregates grew more slowly, kept activity for longer period than adhesive cells （72. 5% vs 47.5% , P 〈 0. 05 ）. Floating aggregates expressed higher level of CD133, CD34 and CD44 than adhesive cells （68.97%, 82.76%, 93.10% vs 5.26%, 15.79%, 5.26%, P 〈 0. 01 ）. Conclusions The combination of CD133, CD34 and CD44 probably can be used as surface markers of cancer stem cells for human lung adenocarcinoma.
作者 谭思创 王若星 谭斯品 胡文 马宇超 喻风雷
机构地区 中南大学湘雅二医院心胸外科 中南大学基础医学院病理生理学系
出处 《中国医师杂志》 CAS 2011年第5期592-595,共4页 Journal of Chinese Physician
基金 教育部博士点基金资助（20070533066）
关键词 肺肿瘤/免疫学 肿瘤干细胞测定 抗原 CD34/代谢 抗原 CD44/代谢 抗 原 CD/代谢 Lung neoplasms/IM Tumor stem cell assay Antigens, CD34/ME Antigens, CD44/ oME Antigens, CD/ME
分类号 R734.2 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1Lapidot T,Sirard C,Vormoor J,et al.A cell initiating human acute myeloid leukaemia after transplantation into SCID mice.Nature,1994,367(6464):645-648.
  • 2Eramo A,Lotti F,Sette G,et al.Identification and expansion of the tumorigenic lung cancer stem cell population.Cell Death Differ,2008,15(3):504-514.
  • 3董强刚,姚明,耿沁,周瑾,闫明霞.人肺腺癌干细胞的分离及鉴定[J].肿瘤,2008,28(1):1-7. 被引量：42
  • 4Meng X,Li M,Wang X,et al.Both CD133+ and CD133-subpopulations of A549 and H446 cells contain cancer-initiating cells.Cancer Sci,2009,100(6):1040-1046.
  • 5Al-Hajj M,Wicha MS,Benito-Hernandez A,et al.Prospective identification of tumorigenic breast cancer cells.Proc Natl Acad Sci U S A,2003,100(7):3983-3988.
  • 6Li C,Heidt DG,Dalerba P,et al.Identification of pancreatic cancer stem cells.Cancer Res,2007,67(3):1030-1037.
  • 7兰勇,魏渝清,贺晓燕.周围型非小细胞性肺癌CD44v6蛋白表达与CT征像的相关性研究[J].中国医师杂志,2006,8(7):883-885. 被引量：2
  • 8Wolf NS,Koné A,Priestley GV,et al.In vivo and in vitro characterization of long-term repopulating primitive hematopoietic cells isolated by sequential Hoechst 33342-rhodamine 123 FACS selection.Exp Hematol,1993,21(5):614-622.
  • 9Kim CF,Jackson EL,Woolfenden AE,et al.Identification of bronchioalveolar stem cells in normal lung and lung cancer.Cell,2005,121(6):823-835.
  • 10Tirino V,Camerlingo R,Franco R,et al.The role of CD133 in the identification and characterisation of tumour-initiating cells in non-small-cell lung cancer.Eur J Cardiothorac Surg,2009,36(3):446-453.

二级参考文献43

  • 1Hefler LA,Concin N,Mincham D,et al.The prognostic value of immunohistochemically detected CD44V3 and CD44V6 expression in patients with surgically staged vulvar carcinoma.Cancer,2002,94(1):125-130.
  • 2Vizoso FJ,Fernandez JC,Corte MD,et al.Expression and clinical significance of CD44V5 and CD44V6 in resectable colorectal cancer.J Cancer Res Clin Oncol,2004,130(11):679-686.
  • 3Faleiro-Rodrigues C,Lopes C.E-cadherin,CD44 and CD44v6 in squamous intraepithelial lesions and invasive carcinomas of the uterine cervix:an immunohistochemical study.Pathobiology,2004,71 (6):329-336.
  • 4Nguyen VN,Mirejovsky T,Melinova L,CD44 and its v6 spliced variant in lung carcinomas:relation to NCAM,CEA,EMA and UP1 and prognostic significance.Neoplasma,2000,47(6):400-408.
  • 5Yu Q,Stamenkovic I.Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion.Genes Dev,1999,13(1):35-48.
  • 6Park CH, Bergsagel DE, McCulloch EA. Mouse myeloma tumor stem cells: Primary cell culture assay [J]. J Natl Cancer Inst, 1971, 46:411-422.
  • 7Lapidot T, Sirard C, Vormoor J, et al. A cellinitiating human acute myeloid leukaemia after transplantation into SC1D mice [J]. Nature,1994, 367: 645-648.
  • 8Clayton H, Titley 1, Vivanco MM. Growth and differentiation of progenitor/stem cells derived from the human mammary gland [J]. Exp Cell Res, 2004, 297(2): 444-460.
  • 9Singh SK, Clarke ID, Terasaki M, et al. Identification of a cancer stem cell in human braintum ors[J]. Cancer Res, 2003, 63: 5821-5828.
  • 10Reva T, Morrison S J, Clarke MF, et al. Stem cells, cancer and cancer stem cells [J]. Nature, 2001, 414: 105-111.

共引文献46

同被引文献79

  • 1Xian W,McKeon F.Adult stem cells underlying lung regeneration[J].Cell Cycle,2012,11(5):887-894.
  • 2Virchow R.Cellular pathology,as based upon physiological andpathological histology.LectureⅩⅥ-Atheromatous affection ofarteries.1858[J].Nutr Rev,1989,47(1):23-25.
  • 3Lapidot T,Sirard C,Vormoor J,et al.A cell initiating human acutemyeloid leukaemia after transplantation into SCID mice[J].Na-ture,1994,367(6464):645-648.
  • 4Shipitsin M,Polyak K.The cancer stem cell hypothesis in search ofdefiniteions,markes,and relevance[J].Lab Invest,2008,88(5):459-463.
  • 5Kim CF,Jackson EL,Woolfenden AE,et al.Identification of bron-chioalveolar stem cells in normal lung and lung cancer[J].Cell,2005,121(6):823-835.
  • 6Tirino V,Camerlingo R,Franco R,et al.The role of CD133 in the i-dentification and characterisation of tumour-initiating cells in non-small-cell lung cancer[J].Eur J Cardiothorac Surg,2009,36(3):446-453.
  • 7Xie T,Li L,Li DR,et al.Isolation and identification of side popu-lation cells in human lung adenocarcinoma cell line A549[J].Zhonghua Zhong Liu Za Zhi,2011,33(2):84-90.
  • 8Al-Hajj M,Wicha MS,Benito-Hernandez A,et al.Prospective i-dentification of tumorigenic breast cancer cells[J].Proc Natl AcadSci U S A,2003,100(7):3983-3988.
  • 9Keller PJ,Arendt LM,Skibinski A,et al.Defining the cellular pre-cursors to human breast cancer[J].Proc Natl Acad Sci U S A,2012,109(8):2772-2777.
  • 10Singh SK,Hawkins C,Clarke ID,et al.Idenification of hunman braintumour initiating cells[J].Nature,2004,432(7015):396-401.

引证文献3

二级引证文献6

中国医师杂志
2011年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部