Nrf2在烧伤脓毒症肺中的动态表达研究

Dynamic expression of NF-E2 in lung of burn septic rat

-目的探讨Nrf2在烧伤脓毒症大鼠肺组织中的表达规律。方法将Wistar大鼠分为正常对照组、单纯烫伤组、烫伤复合金葡菌脓毒症组和烫伤复合绿脓杆菌脓毒症组。依不同时相（2、8、24h），分为三个亚组，检测肺脏组织中Nrf2mRNA、Nrf2的蛋白质表达情况。结果正常大鼠肺组织中Nrf2mRNA较高表达（74．0±7．0）；单纯烫伤大鼠肺组织中Nrf2mRNA表达明显下调，分别为34．5±1．9、50．4±2．2、32．1±1．4（t=5．69～14．63，P〈0．01）；金黄色葡萄球菌所致烫伤脓毒症大鼠肺组织中Nrf2mRNA明显下调，分别为53．1±5．0、14．4±1．6、48．5±1．9，其中伤后8h达峰值（t=5．59-29．3，P〈0．01）；绿脓杆菌所致烫伤脓毒症大鼠伤后2hNff2mRNA表达无明显下降（71．0±8．1，P〉0．05），但伤后8、24h均明显下调（24．8±2．1、4．1±2．0，t=21．33，68．1，P〈0．01）。结论Nrf2直接参与了烧伤后免疫应答反应。

Objective To investigate the expression pattern of Nrf2 in the lung of septic rat and preliminary analysis of the role of Nrf2 in the development of sepsis. Methods Wistar rats were used in this study, it was divided into 4 groups, including normal control group, pure burn group, burn with staphylococcus sepsis group, burn with pseudomonas sepsis group. According to the different time intervals such as 2 hours, 8 hours, and 24 hours, it was divided into three sub-group after injection of bacteria. The expression of Nrf2 in the lung at different time intervals was determined. Results Nrf2 mRNA in the lungs of normal rats was high expression （74. 0 ± 7. 0）, Nrf2 mRNA in the lungs of pure burns rats obviously down- regulated, respectively as 34. 5 ±1.9,50. 4 ＋2. 2,32. 1±1.4, （ t =5.69 ± 14. 63, P 〈0. 01 ）. Nrf2 mR- NA in burn sepsis caused by Staphylococcus aureus in lung tissue of rats down-regulated expression, respectively as 53.1 ±5.0,14.4±1.6,48.5 ±l.9,and reached peak at 8 h（t =5.59-29.3, P 〈0.01）. Pseudomonas aeruginosa burn sepsis didnt induced Nrf2 mRNA in the lung tissue, but it showed a down- ward trend at 2h（71.0 ±8. 1, P 〉0. 05）and markedly reduced after 8, 24 h（24. 8 ±2. 1,4. 1±2. 0, t = 21.33,68.1, P 〈0. 01 ）. Conclusions The distribution of Nrf2 mainly localized in immune organ, and it directly took part in the post burn immune response.