摘要
This study examined the effect of nicotine on the expression of mutant p53（mt-p53） in bladder cancer rats.The rat models of bladder cancer were established by infusing N-methyl-nitroso-urea（MNU,10 mg/kg every 2 weeks for 8 weeks） into the bladder.Pathological examination on the bladder was conducted to confirm the establishment of the model.All the bladder cancer rats were randomly divided into an MNU group and 3 nicotine groups.In the nicotine groups,the rats were intragastrically administered nicotine at different concentrations（25,15,5 mg/kg respectively） 3 times per week for 8 weeks.The mt-p53 expression was detected by the immunohistochemical method.The results showed that rat bladder cancer models developed histopathological changes of bladder transitional cell carcinoma.The positive rate of mt-p53 expression in the 3 nicotine groups（25,15,5 mg/kg） was 75.00%,58.33% and 41.67% by the 14th week,respectively,significantly higher than that in the MNU group（33.33%）（all P〈0.05）.The mt-p53 expression rate was positively correlated with the medication dose and time（P〈0.05）.It is concluded that nicotine may play an important role in the development of bladder cancer partially by increasing the expression of mt-p53.
This study examined the effect of nicotine on the expression of mutant p53（mt-p53） in bladder cancer rats.The rat models of bladder cancer were established by infusing N-methyl-nitroso-urea（MNU,10 mg/kg every 2 weeks for 8 weeks） into the bladder.Pathological examination on the bladder was conducted to confirm the establishment of the model.All the bladder cancer rats were randomly divided into an MNU group and 3 nicotine groups.In the nicotine groups,the rats were intragastrically administered nicotine at different concentrations（25,15,5 mg/kg respectively） 3 times per week for 8 weeks.The mt-p53 expression was detected by the immunohistochemical method.The results showed that rat bladder cancer models developed histopathological changes of bladder transitional cell carcinoma.The positive rate of mt-p53 expression in the 3 nicotine groups（25,15,5 mg/kg） was 75.00%,58.33% and 41.67% by the 14th week,respectively,significantly higher than that in the MNU group（33.33%）（all P〈0.05）.The mt-p53 expression rate was positively correlated with the medication dose and time（P〈0.05）.It is concluded that nicotine may play an important role in the development of bladder cancer partially by increasing the expression of mt-p53.
