大黄素对急性放射性肠炎的疗效及抗自由基损伤的实验研究

Therapeutic effect and anti-free radical injury of emodin on acute radiation enteritis

目的探讨大黄素对急性放射性肠炎的疗效及作用机制。方法选用SD大鼠53只,随机分为正常组、模型组、白头翁汤组和大黄素组。采用6MV高能X射线建立急性放射性肠炎大鼠模型,连续灌胃给药7 d,光学显微镜下观察及图像分析仪测定其相关的形态学指标,并测定小肠组织匀浆NO含量、SOD活性及MDA含量。结果 大黄素组大鼠肠组织绒毛高度、隐窝深度、黏膜及全层壁厚度均显著高于模型组(P<0.05),与白头翁汤组比较无明显差异(P>0.05);大黄素组肠组织匀浆NO含量及MDA含量与模型组比较均显著降低(P<0.05),SOD活性则显著升高(P<0.05);与白头翁汤组比较,NO含量显著降低(P<0.05),SOD活性及MDA含量无明显差异(P>0.05)。结论 大黄素可明显升高小肠组织绒毛高度、隐窝深度、黏膜及全层壁厚度,减少组织NO生成,减轻炎症反应及组织过氧化损伤。

Objective To investigate therapeutic effects and mechanism of emodin on rats with acute radiation enteritis. Methods 53 healthy male SD rats were randomly divided into four groups： the normal group, the model group, the emodin group and the Baitouwengtang （BTWT） group. Except for the normal group, all groups were given a single dose of 10Gy 6MV higher-energy x-rays on the abdominal region to establish acute radiation enteritis models. After radiation, all the rats were intragastricly administered daily. After 7 days of intragastric administration, 10 mL ileum in the ileocecal junction was collected as samples under the condition of general anesthesia. Morphological indices were measured by using a light microscope and image analysis system. Intestinal nitric oxide （NO） levels, superoxide dismutase （SOD） activities and malondialdehyde （MDA） levels were measured by a spectrophotometer. Results The emodin group had more significantly increased height of villus, depth of crypt, and thickness of mucosa and entire wall than the model group did（P 〈0.05）, while there were no differences between the emodin group and the BTWT group （ P 〉 0.05 ）. The emodin group had clearly decreased NO and MDA levels and clearly increased SOD activities in the intestine compared with the model group（ P 〈 0.05 ）. The emodin group had clearly decreased NO levels compared with the BTWT group, while there was no difference in MDA levels and SOD activities between the emodin group and the BTWT group. Conclusion Emodin can relieve intestinal mucosa damage in acute radiation enteritis model rats, reduce NO and MDA generation in the intestinal tissue, and improve SOD activity in the intestinal tissue.