干扰素-γ对小鼠白血病细胞株FBL-3体外侵袭能力影响

Effect of interferon-γ on the invasive capacity of leukemia cell line FBL-3

目的:探讨干扰素-γ不同剂量、不同作用时间对小鼠白血病细胞FBL-3体外侵袭能力的影响。方法:小鼠白血病细胞株FBL-3分为6组,其中A组为空白对照组;B^E组分别用干扰素-γ5,50,200,500 ng/mL处理48 h;F组为干扰素-γ50 ng/mL处理2周。应用Transwell侵袭模型检测各组FBL-3细胞的体外侵袭力,采用单因素方差分析进行比较。结果:与A组比较,C、D和E组穿过Matrigel胶的FBL-3细胞数目明显减少,且存在剂量依赖性效应(P<0.05)。F组细胞侵袭能力增强,明显高于对照组(P<0.05)。结论:干扰素-γ短期作用(48 h)可以剂量依赖性方式明显抑制FBL-3细胞的体外侵袭能力;小剂量干扰素-γ长期作用可促进FBL-3细胞的侵袭能力。

Objective To investigate the effect of interferon-γ on the invasive capacity of leukemia cell line FBL-3 in mice.Methods FBL-3 cells were divided into 6 group in mice with leukemia.Group B（interferon-γ 5 ng/mL）,group C（interferon-γ 50 ng/mL）,group D（interferon-γ 200 ng/mL）,and group E（interferon-γ 500 ng/mL） were treated for 48 hours.Group F（interferon-γ 50 ng/mL） was treated for consecutive 2 weeks.Group A was blank control group.The invasive capacity of FBL-3 in each group were assayed with Transwell membrane invasion culture system,and one-way ANOVA analysis was used to analyze the results.Results Compared with group A,the numbers FBL-3 cells passing through Matrigel in group C,D and E were significantly fewer and dose-dependent（P〈0.05）.On the contrary,the invasive capacity of FBL-3 cell was remarkably enhanced in group F compared with group A.Conclusion Interferon-γ can inhibit the invasive capacity of the FBL-3 cell in a short period（48 hours） in dose-dependent manner,but the consecutive effects（2 weeks） of low dose interferon-γ（50 ng/mL） can significantly promote FBL-3 cell invasiveness.