非胶原蛋白模拟多肽E8DS促进Ⅰ型胶原仿生矿化

Biomineralization of Type Ⅰ Collagen Promoted by an Engineered Non-collagen Protein-derived Peptide E8DS

通过分析骨涎蛋白和牙本质基质蛋白的功能域,设计合成了一种非胶原蛋白模拟多肽E8DS(EEEEEEEEDSESSEEDR),引入胶原蛋白仿生矿化体系,共同调控磷酸钙晶体的矿化过程。圆二色谱和红外光谱分析结果表明,多肽E8DS可与钙离子和胶原分子通过静电作用相结合。使用稳态凝胶系统对多肽E8DS的分析结果表明,E8DS具有很强的调控钙磷盐矿化的能力。多肽的加入有助于胶原纤维的分子组装,增加了形核位点,促进了磷酸钙在胶原纤维表面矿化,使胶原纤维的矿化程度明显提高。

Peptides with sequence（EEEEEEEEDS_pES_pS_pEEDR） were synthesized to mimic the biomineralization function of non-collagenous protein over the typeⅠcollagens.The results show that the peptide can be bound to Ca^（2＋） and typeⅠcollagen by electrostatic interactions.Moreover,synthetic peptide is conducive to calcium ions binding and promotes the nucleation and formation of minerals on the surface of collagen fibrils.Designed peptides also presented a function of accelerating typeⅠcollagen fibrillogenesis and the formation of fibrils bundle or network.