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不同刺激因子对人气道平滑肌细胞白细胞介素-22R1表达的影响

Change of IL-22R1 expression in human airway smooth muscle cells in response to different stimulating agents
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摘要 目的探讨哮喘血清、地塞米松、白细胞介素-4(IL-4)、干扰素γ(IFN-γ)、转化生长因子-β(TGF-β)等不同刺激因子对人气道平滑肌细胞白细胞介素-22R1(IL-22R1)mRNA及蛋白表达的影响。方法用免疫荧光PCR检测不同因素刺激下人气道平滑肌细胞IL-22R1mRNA的表达,用Westernblot检测人不同因素刺激下的人气道平滑肌细胞IL-22R1蛋白的表达,比较不同组别之间的差异。结果哮喘血清刺激下的人气道平滑肌细胞IL-22R1mRNA升至对照组345倍,而蛋白表达明显增强(P<0.01);加用地塞米松后IL-22R1mRNA降至对照组10倍,蛋白表达较仅用哮喘血清刺激明显减弱(P<0.01)。IL-4、IFN-γ、TGF-β刺激后人气道平滑肌细胞IL-22R1mRNA及蛋白表达均显著性增加。结论 IL-22R1表达的改变可能参与支气管哮喘的病程,影响人气道平滑肌细胞IL-22R1受体的表达可能是地塞米松发挥其在支气管哮喘病程中作用的机制之一。IFN-γ、IL-4、TGF-β对气道平滑肌细胞IL-22R1的作用可能是其对支气管哮喘疾病产生影响的机制之一。 Objective To explore the effects of serum of asthmatic patients, dexamethasone, interleukin-4 （IL-4）, interferon-gamma （IFN-γ） and transforming growth factor-β （TGF-β） on the expression of interleukin-22 receptor 1 （IL-22R1） mRNA and protein in HASMCs in vitro. Methods IL-22R1 mRNA and protein expressions in HASMCs treated with different stimulating agents were measured by real-time PCR and Western blotting, respectively. Results IL-22R1 mRNA and protein expressions in HASMCs were significantly increased after stimulation by serum from asthmatic patients, but decreased after co-stimulation with dexamethasone. IL-22R1 mRNA and protein expressions in the cells both increased after stimulation by IL-4, IFN-γ and TGF-β. Conclusion IL-22R1 in HASMCs might be involved in the pathogenesis of asthma, and the therapeutic effect of dexamethasone on asthma is mediated, at least partially, by IL-22R1. The effects of IFN-γ, IL-4, and TGF-β on asthma may also be attributed to their actions on HASMCs
作者 张旃 罗雅玲 周丽丽 赖文岩 徐建 黎振兴 任敦强 叶涵 钟浩海
机构地区 南方医科大学南方医院呼吸内科 南方医科大学南方医院肾内科 南方医科大学南方医院心血管内科重点实验室 南方医科大学南方医院肾移植科
出处 《南方医科大学学报》 CAS CSCD 北大核心 2011年第6期979-982,共4页 Journal of Southern Medical University
基金 国家自然科学基金(30770936)~~
关键词 人气道平滑肌细胞白细胞介素-22R1 人气道平滑肌细胞 白细胞介素-4 干扰素Γ 转化生长因子-Β interleukin-22 receptor 1 human airway smooth muscle cells interleukin-4 interferon-γ transforminggrowth factor-β
分类号 R562.25 [医药卫生—呼吸系统]
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参考文献13

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南方医科大学学报
2011年 第6期

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