自发性2型糖尿病大鼠血浆低脂联素血症导致胰岛素抵抗

Hypoadiponectinemia leads to insulin resistance in OLETF rats: a preliminary study

目的观察自发性2型糖尿病大鼠血浆脂联素(APN)与胰岛素抵抗的关系。方法自发性2型糖尿病大鼠OLETF鼠20只,同系健康对照LETO鼠10只作为正常对照组。饲养13和40周后,行口服葡萄糖耐量试验;于8、32和40周分批宰杀,检测血糖、血浆脂联素、胰岛素、血清甘油三酯、游离脂肪酸和胆固醇水平。结果 8周龄时OLETF鼠血浆APN水平显著低于LETO组(P<0.05),并进行性降低。32周龄和40周龄时OLETF组大鼠血浆胰岛素水平和甘油三酯、胆固醇、游离脂肪酸显著高于LETO组(P<0.01)。结论 OLETF鼠血浆APN水平与胰岛素抵抗显著负相关,且先于胰岛素抵抗和葡萄糖耐量受损发生;低脂联素血症可能是导致胰岛素抵抗的重要原因。

Objective To investigate the association between plasma adiponectin and insulin resistance in OLETF rats. Methods Twenty male Otsuka Long-Evans Tokushima Fatty （OLETF） rats and 10 male Long-evans Tokushima Otsuka （LETO） rats underwent oral glucose tolerance test （OGTT） at 13 and 40 weeks of age. At 8, 32 and 40 weeks of age, the rats were sacrificed to measure the blood glucose, plasma insulin and adiponectin levels, and serum levels of TG, CHOL and FFA. Results The plasma adiponectin level was significantly decreased in 8-week-old OLETF rats compared with that of LETO rats （P〈0.05）. The plasma insulin level, TG, CHOL, and FFA were significantly higher in OLETF rats than in LETO rats at 32 and 40 weeks of age. Conclusion A decreased plasma level of adiponectin preludes insulin resistance and is inversely correlated to insulin sensitivity. Hypoadiponectinemia may be an important reason leading to insulin resistance