模拟微重力对单核细胞增殖及组织因子mRNA表达的影响

Effect of simulated microgravity on human monocytic cell proliferation and tissue factor mRNA expression

目的探讨模拟微重力对单核细胞增殖及组织因子(TF)mRNA表达的影响。方法将单核细胞株THP-1细胞置于RCCS系统中进行培养,以细胞计数和流式细胞术测定THP-1细胞的增殖能力;RT-PCR检测THP-1细胞TFmRNA的表达。结果模拟微重力条件下THP-1细胞计数显著低于静止组(P<0.01)。培养24h后,微重力组G0/G1期细胞(67.64±2.71)%明显高于静止组(46.57±1.64)%(P<0.05)。THP-1细胞未受LPS刺激时,微重力组和静止组均低表达TFmRNA,两者间无统计学差异;加入LPS培养4h后,微重力组和静止组THP-1细胞TF mRNA表达均增加,但微重力组(2.301±0.179)明显低于静止LPS刺激组(9.210±1.328)(P<0.05)。结论模拟微重力可抑制单核细胞的增殖以及LPS刺激下TFmRNA表达的增加。

Objective To investigate the effect of simulated microgravity on the proliferation of human monocytic cells THP-1 and the expression of tissue factor （TF） mRNA. Methods THP-1 cells were cultured under a simulated microgravity environment using the rotating cell culture system （RCCS）. The changes in the cell proliferation after microgravity culture were assessed by cell counting and cell cycle analysis with flow cytometry. RT-PCR was used to detect the changes in the expression of TF mRNA in THP-1 cells. Results Culture under simulated microgravity resulted in a significant decrease in the cell number of THP-1 cells in comparison with that of the control cells （P〈0.01）. After a 24-h culture under microgravity, the G0-Gl phase cells increased from the control level of （46.57±1.64）% to （67.64±2.71）% （P〈0.05）. The cells in both groups showed a low level of TF mRNA expression in the absence of LPS stimulation. A 4-h stimulation with LPS caused up-regulated expression of TF mRNA in both cells, but the microgravity group showed a significantly smaller increase in the expression （2.301±0.179） than the control group （9.210±1.328） （P〈0.05）. Conclusion Microgravity can inhibit the proliferation of THP-1 cells and suppress the cellular expression of TF mRNA