三七醇提物对大鼠肝组织体内外抗氧化作用的研究

The antioxidative activities of panax notoginseng in rat livers in vivo and in vitro

目的:研究三七(panax notoginseng,PNG)醇提物对大鼠肝组织的体内外抗氧化作用。方法:采用羟自由基(·OH)和超氧阴离子自由基(O_2^-)两种化学发光体系,观察三七醇提物对化学发光强度的抑制作用;采用过氧基异丙苯(cumene hydroperoxide,CHP)、维生素C(V_c)/硫酸亚铁(Fe^(2+))和四氯化碳(CCl_4)/还原型辅酶Ⅱ(NADPH)作为激发剂,建立3种微粒体脂质过氧化(lipid peroxidation,LPO)损伤模型,观察三七醇提物对内二醛(MDA)生成的抑制作用。用^(60)Coγ射线辐照大鼠建立氧化损伤模型,观察三七醇提物对肝组织的氧化型谷胱甘肽(GSSG)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)含量及活性的影响,以及对MDA生成的抑制作用。结果:在两种化学发光的体系中,三七醇提物对O_2^-和·OH的抑制率均显著高于阴性对照组(P<0.05),且呈明显剂量-效应关系,三七醇提物对O_2^-和·OH的IC_(50)分别为1.25和0.625/mg/ml;3种LPO损伤模型中三七醇提物不同浓度组的MDA含量均显著低于对照组(P<0.05);在体大鼠氧化损伤模型实验的肝组织中,三七醇提物不同剂量灌胃组[(50、225和450mg/(kg·d)],其MDA的含量均较阳性对照组(仅做^(60)Coγ照射)降低(P<0.05);225和450mg/(kg·d)剂量组CAT活性较阳性对照组显著升高(P<0.05);其450mg/(kg·d)剂量组SOD的活性较阳性对照组显著升高(P<0.05);而225和1450mg/(kg·d)剂量组GSSG含量较阳性对照组显著降低(P<0.05)。结论:三七醇提物对体外氧化损伤模型具有很强的抗氧化作用,对辐射引起的大鼠体内肝脏氧化损伤具有一定的抗氧化修复功效,并呈剂量-效应关系。

OBJECTIVE：To study the antioxidative activities of the ethanol extract of panax notoginseng （PNG）in several free radical models in vitro and in oxidative damage rat model in vivo.METHODS：The chemiluminescence systems models for determination of hydroxyl radical（·OH）and superoxide anion radical（O_2^-）were built to observe the inhibitory rates of luminous intensity by different concentrations of PNG extract.The microsomal lipid peroxidation model stimulated by CHP,V_C/Fe^（2＋）and CCl_4/NADPH were established to observe the inhibition of MDA by different concentrations of PNG extract.The oxidative damage rat model was built by ^（60）Co-γray to observe the inhibitory effect of PNG extract on the generation of MDA and GSSG,and the changes of CAT and SOD activities in livers. RESULTS：In the two chemiluminescence systems,the inhibitory rates of ？OH and O_2^-by PNG extract were significantly higher than that in control group,with significant dose-effect relationship.The IC_（50）of O_2^-and ·OH by ethanol extract of PNG were 1.25 mg/ml and 0.625 mg/ml,respectively.Compared with that in control group,the content of MDA in the three LPO models decreased significantly（P0.05）at different concentrations,with a dose-effect relationship.After PNG oral administration,decrease of MDA was observed in rat livers.The activities of SOD and CAT were increased by ethanol extract,the content of GSSG was decreased by ethanol extract.CONCLUSION：In several in vitro and in vivo models described above,the ethanol extract of PNG demonstrated effective antioxidative effects and revealed a dose-effect relationship.