Zwint-1及其变异体Zwint-1v在HeLa细胞不同细胞周期中的亚定位

The sub-localization of Zwint-1 and Zwint-1v in different cell cycles of HeLa cells

目的:探讨Zw10结合因子-1(zeste white 10 interactor-1,Zwint-1)及其变异体(Zwint-1v)在HeLa细胞不同细胞周期中的亚定位。方法:采用胸腺嘧啶核苷双阻断法将HeLa细胞同步化,通过瞬时转染及间接免疫荧光检测,观察Zwint-1及Zwint-1v在HeLa细胞不同细胞周期中的亚定位。结果:在HeLa细胞间期,Zwint-1野生型定位于细胞质,而Zwint-1v定位于细胞核;在分裂期,Zwint-1野生型及Zwint-1v定位于纺锤体与着丝粒上。Zwint-1v定化较野生型更趋近于细胞核。结论:Zwint-1及Zwint-1v在HeLa细胞不同细胞周期的亚定位存在差异。

OBJECTIVE：To study the sub-localization of Zwint-1 wild type and variant（Zwint-1v）in different cell cycles of HeLa cells.METHODS：HeLa cells were synchronized using thymidine double-block method. The sub-localization of Zwint-1 wild type and Zwint-1v were identified by fluorescence microscope after transfection and indirect immunofluorescence.RESULTS：In interphase of HeLa cells,Zwint-1 wild type was localized in the cytoplasm,while Zwint-1v were in nucleus.In mitotic phase,Zwint-1 wild type and Zwint-1v were found on the spindle and centromere.Zwint-1v was closer to the nucleus than the wild type.CONCLUSION：Zwint-1 wild type and Zwint-1v during cell cycle have different sub-localization.