胡桃楸水提物的遗传毒性及抗诱变作用研究

Study on the genetoxic and anti-mutagenesis effects of juglans mandshurica maxim extract

目的:探讨胡桃楸水提物(juglans mandshurica maxim extract,JMME)的抗诱变作用,及评价胡桃楸是否具有遗传毒性。方法:取80只昆明小鼠,随机分成不同JMME剂量的诱变组(6.55、13.09、26.18g/kg,分别为1/8、1/4、1/2 LD_(50)),不同剂量的JMME与环磷酰胺(cyclophosphamide,CP)(0.04g/kg)联合应用的抗诱变组,阴性对照组(NS)和阳性对照组(CP,0.04g/kg),共8组,每组10只。采用小鼠骨髓细胞微核试验(MNT)检测JMME是否具有遗传毒性及抗诱变作用。结果:阳性对照组微核发生率(28.82‰)明显高于阴性对照组(2.65‰),其差异具有统计学意义(P<0.01),而JMME各剂量组与阴性对照组间的差异无统计学意义(P>0.05)。抗诱变各组的微核发生率与阳性对照组比较,除JMME低剂量组(6.55g/kg)无统计学意义外(P>0.05),JMME中剂量组(13.09g/kg)和高剂量组(26.18g/kg)均显著低于阳性对照组(P<0.05),其中高剂量组明显低于中剂量组(P<0.05)。结论:在本实验条件下,JMME无遗传毒性,JMME对CP诱发的微核率,具有一定抵抗作用,且呈量-效关系。

OBJECTIVE：To discuss the mutagenesis and anti-mutagenesis effects of juglans mandshurica maxim extract（JMME）and to evaluate whether juglans mandshurica is genotoxie or not.METHODS：To choose 80 KM mice,and randomly grouped negative control group,positive control group（CP,0.04 g/kg）,water extract groups with different dose JMME（6.55,13.09,26.18 g/kg,1/8,1/4,1/2 LD_（50））,and antimutagenieity groups with different dose JMME water extract and CP（0.04 g/kg）.There were 8 groups with 10 mice each group.Test whether the JMME had genotoxicity and anti-mutagenic effect,marrow micronuclei test was carried out.RESULTS：The micronucleus incidence of negative control group was 2.65‰,and that of positive control group was 28.82‰（P0.01）.But there were little statistical differences（P0.05）between the water extract groups with different doses JMME and the negative control group.Comparing the antimutagenicity groups with the positive control group,the low dose（6.55 g/kg）group showed no statistical significance（P0.05）,but both the intermediate dose（12.09 g/kg）group and the high dose（26.18 g/kg）group had significant difference（P0.05）,and the high dose group was lower than the intermediate group（P0.05）. CONCLUSION：Under our experimental conditions,JMME had no genotoxicity.For the micronucleus incidence induced by CP,it had some dose-dependent anti-mutagenesis effect.