天绿香体外安全性毒理学评价研究

Safety assessment of Sauropus androgynus L Merr.in vitro

目的:对野生蔬菜天绿香匀浆液的细胞毒性和遗传毒性进行评价。方法:天绿香匀浆液分别以1450、725、363、181、90.6、45.3、22.6、11.3mg/ml浓度对人胚肺细胞MRC-5及人肝肿瘤细胞HepG2染毒,24h后以中性红摄入法对天绿香匀浆液的细胞毒性进行测定,计算IC_(50);根据细胞毒性结果,以72.5、36.3、18.1mg/ml天绿香匀浆液处理HepG2细胞,并同时设阴性对照组(仅加DMEM培养液)和阳性对照组[B(a)P],进行体外微核分析,记录微核细胞数,计算微核率。结果:染毒24h后观察到天绿香匀浆液对MRC-5细胞的IC_(50)为133.1mg/ml,而对Hep G2细胞的IC_(50)为185.1mg/ml,天绿香匀浆液对肺来源细胞的毒性明显大于肝来源的细胞。不同浓度天绿香匀浆液染毒的HepG2细胞微核率与阴性对照组比较,差异均无统计学意义(P>0.05)。结论:天绿香匀浆液对细胞的损伤作用具有一定的组织选择性,对人肺组织来源的细胞损伤作用大于肝组织来源的细胞。未发现到天绿香对HepG2细胞遗传物质具有损伤作用。

OBJECTIVE：The cytotoxicity and genotoxicity of Sauropus androgynus L.Merr.（SA）was assessed using human cell lines MRC-5 and Hep G2 in vitro.METHODS：MRC-5 and Hep G2 cells were treated with uncooked SA homogenate at 1450,725,363,181,90.6,45.3,22.6,11.3 mg/ml for 24 h.Then the cytotoxicity was studied using the neutral red intake test and the 50%inhibition concentration（IC_（50））was obtained.According to the cytotoxicity results,Hep G2 cells were treated with SA at 72.5,36.3,18.1 mg/ml,and in vitro micronuclei（MN） incidence was analysed.The number of cells with MN was recorded and the frequency was calculated.RESULTS：IC_（50） was 133.1 mg/ml in MRC-5 cells and 185.1 mg/ml in Hep G2 cells which were all treated for 24 h with SA.The cytotoxicity of SA on MRC-5 cells originated from human lung was more severe than Hep G2 cells originated from human liver.There was no significant difference between the groups of various SA concentrations and the negative control（P0.05） in Hep G2 cell micronuelei test.CONCLUSION：SA homogenate could selectively damage MRC-5 cells originated from human lung,compared to Hep G2 cells originated from human liver.No obvious damage to genetic materials could be observed in Hep G2 cells.