基质细胞衍生因子1在人炎症牙髓组织中表达的实验研究

Expression of Stromal cell-derived factor-1 in human inflamed dental pulp tissue

目的:研究基质细胞衍生因子1(Stromal cell-derived factor-1,SDF-1)及其受体CXCR4在人炎性牙髓组织中的表达,探讨SDF-1/CXCR4轴在牙髓炎症发生发展中的可能作用。方法:采用免疫组织化学染色的方法检测SDF-1和CXCR4阳性细胞在健康、炎症牙髓组织中的分布情况。以实时荧光定量RT-PCR方法检测SDF-1mRNA在健康和炎症牙髓中的表达。结果:炎性牙髓中SDF-1、CXCR4主要分布于炎性细胞、成牙本质细胞和微血管内皮细胞。而正常组牙髓少见SDF-1、CXCR4阳性细胞。炎性牙髓中SDF-1mRNA的表达较健康牙髓显著增强。结论:与正常牙髓相比,炎性牙髓组织中SDF-1、CXCR4阳性细胞明显增多。炎性牙髓中SDF-1表达水平明显上调。SDF-1/CXCR4轴可能参与了牙髓炎症损伤和修复过程。

AIM： To investigate the expression of Stromal cell-derived factor-1 （SDF-1 ） and CXCR4 in human inflammed dental pulps and to explore the role of SDF-1/CXCR4 axis in pulpal inflammation. METHODS ： Inflamed dental pulp tissues were obtained from extracted teeth that showed spontaneous pain and/or lingering pain in response to cold and/or heat stimulus, and normal dental pulp tissues were obtained from healthy noncarious teeth. Distribution of SDF-l-positive and CXCR4-positive cells in the dental pulp tissues was investigated by immunohistochem- istry. Real time RT-PCR was performed to detect SDF-1 mRNA expression in healthy and inflamed pulp tissues. RESULTS： In inflamed pulps, SDF-1 and CXCR4 were mostly distributed in inflammatory cells, odontoblasts and microvascular endothelial cells. In contrast, SDF-1 and CXCR4 were scarcely detected in normal pulp tissues. SDF-1 mRNA expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. CONCLUSION： SDF-l-positive and CXCR4-positive cells were significantly increased in inflamed dental pulp tissues as compared with non-inflamed dental pulp tissues. Our findings suggest that SDF-1 plays an important role in the advancement of pulpal inflammation via the recruitment of CXCR4-expressing inflammatory cell and SDF-1/CXCR4 axis maybe involved in the recruitment of dental pulp inflammatory cells at the injury site.