地塞米松对大鼠脑出血后周围脑组织MMP-9表达的影响

Interference of DEX application to the expression of MMP-9 in the area around hematoma in rats:An experimental study

目的探讨地塞米松(DEX)对大鼠实验性脑出血后基质金属蛋白酶-9(MMP-9)表达的影响。方法 63只健康雄性Wistar大鼠采用自体血注入法建立右侧基底节区脑出血模型,随机分为A、B、C 3组,A组为DEX干预组;B组为脑出血模型组;C组为假手术对照组,造模成功后每天给予A组DEX(1 ml/kg)腹腔注射,B、C 2组用等量生理盐水替代,分别于造模后6 h、12 h、24 h、3 d、5 d、7 d、14 d 7个时间点取脑,免疫组化检测MMP-9的表达。结果 A组MMP-9表达较B组降低(P<0.05),A、B 2组MMP-9表达较C组升高(P<0.05)。结论 DEX能降低大鼠脑出血后血肿周围脑组织MMP-9的表达,MMP-9表达的变化可能为DEX减轻脑水肿的作用通道之一。

Objective To study the relationship between the expression of matrix metalloproteinase-9（MMP-9） around the hematoma and the application of dexamethasone（DEX） after intra-cerebral hemorrhage（ICH）.Methods Sixty-three healthy male Wistar rats were randomly divided into three groups：ICH with DEX treatment group（group A）,ICH group（group B） and sham operation group（group C）.The model of ICH in right ganglia was established by autologous blood injection.After modeling,the rats of group A were given a daily intra-peritoneal injection of DEX（1ml/kg）.Groups of B and C were given equivalent normal saline instead.After intervention,the rats were decapitated respectively,at time points of 6h,12h,24h,3d,7d and 14d.The expression of MMP-9 was measured by immunohistochemistry technique.Results The expression of MMP-9 in group A was lower than that in group B（P0.05）.The expression of MMP-9 in groups A and B was higher than that in group C（P0.05）.Conclusion The application of DEX can decrease the expression of MMP-9 in the area around hematoma.The change in the expression of MMP-9 may be used as a channel to alleviate cerebral edema.