细菌毒力基因的筛选技术研究进展

Reserch Progress on Filtration Technologies of Bacterial Virulence Gene

细菌感染宿主是一个复杂的过程。目前对于细菌的致病机制的研究主要集中在细菌毒力因子的鉴定上,主要通过筛选细菌感染宿主过程中特异表达的毒力基因,探讨其与细菌毒力之间的关系,来达到识别和确定细菌毒力因子的目的。近年来建立的标记突变技术、体内表达技术、差异荧光诱导技术和体内诱生抗原鉴定技术,可高通量地筛选出宿主体内特异表达的已知或者未知的细菌毒力基因。现对目前运用的细菌毒力基因筛选技术的原理及优缺点进行综述。

The process of bacterial infection are very complex.At present,the study on the pathogenesis of bacterial infection was mainly foucussed on the identification of virulence factors.To identify the virulence factors,it is necessary to screen the virulence genes which are specifically induced in host during bacterial infection and discuss the interaction between the virulence genes and the bacterial virulence.In recent years,different filtration technologies were developed to analyze and identify the knowned and unknowned bacterial virulence gene which could be screened with high throughput in vivo,including signature tagged mutagenesis,In vivo expression technology,differential fluorescence induction,and in vivo-induced antigen technology.The principle,advantages and disadvantages of these technologies were reviewed in this article.